Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.

Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.

A messenger ribonucleic acid (mRNA) vaccine for Bandavirus dabieense (DBV) and a preparation method thereof are provided. The provided mRNA molecule is an mRNA obtained by cloning an optimized DBV glycoprotein gonadotropins (Gn) sequence into a pGEM-3Zf (+) mRNA vaccine vector, linearizing a plasmid via enzyme digestion, and capping and adding a poly(A) tail through an in vitro transcription enzyme method. The mRNA is encapsulated using a lipid nanoparticle delivery system to obtain the mRNA vaccine.