Among other things, the present disclosure provides biosynthesis polypeptides, methods, and non-naturally occurring microbial organisms for preparing various compounds such as 1,5-pentanediol, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, and 2-keto carboxylic acids.

This disclosure is related to a process and apparatus for producing and recovering at least one fermentation product from a fermentation process using a C1-containing gas passed to a fermentation bioreactor, that produces a fermentation broth comprising at least one of a first product stream comprising ethanol and water or a second product stream comprising ethanol, acetone, and water or a third product stream comprising ethanol, acetone, isopropanol, and water. The product is recovered by using a shared product recovery system. Particularly, the shared product recovery system selectively recovers at least one enriched product stream selected from an enriched ethanol stream, an enriched acetone stream, an enriched isopropanol stream or combinations thereof. The shared product recovery system includes at least one of a vacuum distillation unit, a rectification unit, an acetone removal unit, a drying unit, an ethanol-acetone separation unit, an extractive distillation unit or combinations thereof.

The application generally relates to bioproduction of molecules of interest in microorganisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to express or overexpress a mutant of a bubblegum-type acyl-CoA synthase, and uses thereof.

The application generally relates to bioproduction of molecules of interest in microorganisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to express or overexpress a mutant of a bubblegum-type acyl-CoA synthase, and uses thereof.

A system for the production of high value chemicals includes (a) an input selected from the group consisting of ethylene glycol, glycerol, ethanol methanol or a combination thereof. In addition, the system includes (b) an oxidation biocatalyst including an alcohol oxidase, a copper radical oxidase, a glycerol oxidase, an alditol oxidase or a combination thereof. Further, the system includes (c) an oxidized intermediate. The system also includes (d) a finishing catalyst including a supported metal catalyst, a carboligating catalyst, an amine oxidase, a glyoxalase, an acid catalyst, a base catalyst, an isomerization catalyst or a combination thereof. Still further, the system includes (e) an output.

The present disclosure provides a conversion of a gaseous substrate with the use of a microorganism. In one aspect, the present disclosure provides a microbial gas-phase reaction system for converting a gaseous substrate with the use of a microorganism. This microbial gas-phase reaction system comprises at least one member selected from among a carrier having the microorganism immobilized thereon, a gas supply part for supplying the gaseous substrate to the gas phase of the microbial gas-phase reaction system, and a water supply system for supplying water to the carrier. In one aspect, the present disclosure provides a method for converting a gaseous substrate with the use of a microorganism. This method comprises a step for exposing a surface of a carrier, on which the microorganism is immobilized, to a gas phase containing the gaseous substrate.

The present invention relates to the production of compounds comprised in pheromones, in particular moth pheromones, such as desaturated fatty alcohols and desaturated fatty acyl acetates and derivatives thereof, from a yeast cell.

The present invention relates to the production of compounds comprised in pheromones, in particular moth pheromones, such as desaturated fatty alcohols and desaturated fatty acyl acetates and derivatives thereof, from a yeast cell.

Modification of the amino acid sequence of a phenylpyruvate decarboxylase from Azospirillum brasilense produces a novel group of phenylpyruvate decarboxylases with improved specificity to certain substrates, including in particular C7-C11 2-ketoacids such as, for example, 2-ketononanoate and 2-keto-octanoate. This specificity enables effective use of the phenylpyruvate decarboxylase in, for example, an in vivo process wherein 2-ketobutyrate or 2-ketoisovalerate are converted to C7-C11 2-ketoacids, and the novel phenylpyruvate decarboxylase converts the C7-C11 2-ketoacid to a C6-C10 aldehyde having one less carbon than the 2-ketoacid. Ultimately, through contact with additional enzymes, such C6-C10 aldehydes may be converted to, for example, C6-C10 alcohols, C6-C10 carboxylic acids, C6-C10 alkanes, and other derivatives. Use of the novel genetically modified phenylpyruvate de carboxylases may represent a lower cost alternative to non-biobased approaches.

Modification of the amino acid sequence of a phenylpyruvate decarboxylase from Azospirillum brasilense produces a novel group of phenylpyruvate decarboxylases with improved specificity to certain substrates, including in particular C7-C11 2-ketoacids such as, for example, 2-ketononanoate and 2-keto-octanoate. This specificity enables effective use of the phenylpyruvate decarboxylase in, for example, an in vivo process wherein 2-ketobutyrate or 2-ketoisovalerate are converted to C7-C11 2-ketoacids, and the novel phenylpyruvate decarboxylase converts the C7-C11 2-ketoacid to a C6-C10 aldehyde having one less carbon than the 2-ketoacid. Ultimately, through contact with additional enzymes, such C6-C10 aldehydes may be converted to, for example, C6-C10 alcohols, C6-C10 carboxylic acids, C6-C10 alkanes, and other derivatives. Use of the novel genetically modified phenylpyruvate de carboxylases may represent a lower cost alternative to non-biobased approaches.