The present disclosure discloses Stenotrophomonas pavanii capable of degrading polyethylene terephthalate, belonging to the technical field of microorganisms. The present disclosure provides a S. pavanii strain JWG-G1 capable of degrading polyethylene terephthalate (PET). After a seed solution of the S. pavanii is inoculated into an inorganic salt liquid medium containing 2 g/L polyethylene terephthalate at an inoculum size of 10% (v/v) and cultured for 5 d, polyethylene terephthalate (PET) particles can be partially degraded into monohydroxyethyl terephthalate and terephthalic acid that can be directly recycled. In addition, ester bond functional groups on the surface of the polyethylene terephthalate plastic particles can be reduced, and a weight loss rate of the polyethylene terephthalate plastic particles can reach 9.4%. Therefore, the S. pavanii JWG-G1 of the present disclosure has very high application prospects in the degradation of polyethylene terephthalate.

The present disclosure discloses Stenotrophomonas pavanii capable of degrading polyethylene terephthalate, belonging to the technical field of microorganisms. The present disclosure provides a S. pavanii strain JWG-G1 capable of degrading polyethylene terephthalate (PET). After a seed solution of the S. pavanii is inoculated into an inorganic salt liquid medium containing 2 g/L polyethylene terephthalate at an inoculum size of 10% (v/v) and cultured for 5 d, polyethylene terephthalate (PET) particles can be partially degraded into monohydroxyethyl terephthalate and terephthalic acid that can be directly recycled. In addition, ester bond functional groups on the surface of the polyethylene terephthalate plastic particles can be reduced, and a weight loss rate of the polyethylene terephthalate plastic particles can reach 9.4%. Therefore, the S. pavanii JWG-G1 of the present disclosure has very high application prospects in the degradation of polyethylene terephthalate.

Among other things, the present disclosure provides biosynthesis polypeptides, methods, and non-naturally occurring microbial organisms for preparing various compounds such as 1,5-pentanediol, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, and 2-keto carboxylic acids.

Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as α-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a C.sub.N aldehyde and pyruvate to a C.sub.N+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the C.sub.N+3 β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as α-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a C.sub.N aldehyde and pyruvate to a C.sub.N+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the C.sub.N+3 β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

Among other things, the present disclosure provides biosynthesis polypeptides, methods, and non-naturally occurring microbial organisms for preparing various compounds such as 1,5-pentanediol, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, and 2-keto carboxylic acids.

A long-chain composition has at least one long-chain alkane selected from the group consisting of C9-18 linear or branched alkanes and at least one long-chain carboxylic acid selected from the group consisting of C9-18 linear or branched, saturated or unsaturated aliphatic monocarboxylic acids. The mass ratio of the long-chain alkane to the long-chain carboxylic acid ranges from 1:1 to 40:1. The long-chain composition has a higher fermentation degree or higher substrate utilization rate and the like, when used as a starting material in the production of long-chain dibasic acids via fermentation.

The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.

The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.

The present invention relates to a method of producing adipic acid, including a step (hydrogenation step) of reacting 3-hydroxyadipic acid-3,6-lactone with hydrogen in an aqueous solvent in a presence of a hydrogenation catalyst. The hydrogenation catalyst preferably includes one kind or two or more kinds of transition metal elements selected from the group consisting of palladium, platinum, ruthenium, rhodium, rhenium, nickel, cobalt, iron, iridium, osmium, copper, and chromium.