The present disclosure relates to a method that includes utilizing a microorganism for the converting of a substrate to an acid contained in a mixture that includes the acid and water, maintaining a pH of the mixture to less than 5, and treating the mixture with a first stream comprising an organic.

The invention relates to a method for producing a sugar syrup comprising fermentable sugars from lignocellulosic biomass containing paper waste, in particular printable paper, printed paper or cardboard, said method comprising the following steps: a. optionally, a step of shredding said lignocellulosic biomass containing paper waste; b.i. a step of impregnating said lignocellulosic biomass containing paper waste or shredded lignocellulosic biomass obtained on completion of step a. in an aqueous medium, and ii. a thermal pretreatment step implemented, without the addition of acid, at a temperature of between 80° C. and 150° C., at a pH between 6.5 and 8.5, in particular between 6.5 and 8, in order to obtain a pretreated product, said impregnation and thermal pretreatment steps being carried out simultaneously or successively according to i. and then ii; c. a step of enzymatic hydrolysis of the pretreated product obtained on completion of step b. in order to convert the cellulose and hemicellulose into a sugar syrup comprising fermentable sugars; and d. a step of recovering the sugar syrup comprising fermentable sugars obtained on completion of step c.

A method of producing chemicals includes providing fermentative cells; co-feeding any of galacturonate and galacturonate polymers with carbohydrates to the fermentative cells; and producing a chemical from the fermentative cells. The fermentative cells may include any of Clostridium acetobutylicum and Clostridium saccharoperbutylacetonicum. The carbohydrates may include any of glucose, mannose, galactose, fructose, arabinose, xylose, sucrose, lactose, maltose, cellobiose, and starch. The method may include providing a substantially equal proportion of the any of galacturonate and galacturonate polymers and the carbohydrates for co-feeding to the fermentative cells. The method may include altering a proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The method may include modulating a production of the chemical by altering the proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The chemical may include any of acetate and butyrate.

A method of producing chemicals includes providing fermentative cells; co-feeding any of galacturonate and galacturonate polymers with carbohydrates to the fermentative cells; and producing a chemical from the fermentative cells. The fermentative cells may include any of Clostridium acetobutylicum and Clostridium saccharoperbutylacetonicum. The carbohydrates may include any of glucose, mannose, galactose, fructose, arabinose, xylose, sucrose, lactose, maltose, cellobiose, and starch. The method may include providing a substantially equal proportion of the any of galacturonate and galacturonate polymers and the carbohydrates for co-feeding to the fermentative cells. The method may include altering a proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The method may include modulating a production of the chemical by altering the proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The chemical may include any of acetate and butyrate.

Bioreactors for production and recovery of medium chain carboxylates from organic biomass are disclosed. Methods for improved production and recovery of medium chain carboxylates from organic biomass are also disclosed. The bioreactors can be used as a chain-elongation bioreactor, and a method of use thereof results in improved production and recovery of medium chain carboxylates from organic biomass. The bioreactor includes a shell defined by one or more walls and a length, and a plurality of porous hollow fiber membranes placed inside the reactor for continuous liquid-liquid extraction, as well as granular activated carbon (GAC) as biocarriers. The plurality of hollow fiber membranes is mounted such that a percentage of the length of the shell remains unoccupied by the plurality of porous hollow fiber membranes.

Bioreactors for production and recovery of medium chain carboxylates from organic biomass are disclosed. Methods for improved production and recovery of medium chain carboxylates from organic biomass are also disclosed. The bioreactors can be used as a chain-elongation bioreactor, and a method of use thereof results in improved production and recovery of medium chain carboxylates from organic biomass. The bioreactor includes a shell defined by one or more walls and a length, and a plurality of porous hollow fiber membranes placed inside the reactor for continuous liquid-liquid extraction, as well as granular activated carbon (GAC) as biocarriers. The plurality of hollow fiber membranes is mounted such that a percentage of the length of the shell remains unoccupied by the plurality of porous hollow fiber membranes.

A CO.sub.2, bioconversion process includes providing a CO.sub.2 containing substrate to a bioreactor, the CO.sub.2 containing substrate including about 5 to about 90 mole % CO.sub.2; and fermenting the CO.sub.2 containing substrate with an acetogenic bacteria carrying a sodium translocating ATPase. The medium including less than about 0.01 grams per liter yeast extract, less than about 0.01 grams per liter carbohydrate, a sodium ion concentration provided by a sodium ion feed rate of about 290 to about 8750 μg/gram of cells/minute, and a pH of about 4 to about 6.9.

A CO.sub.2, bioconversion process includes providing a CO.sub.2 containing substrate to a bioreactor, the CO.sub.2 containing substrate including about 5 to about 90 mole % CO.sub.2; and fermenting the CO.sub.2 containing substrate with an acetogenic bacteria carrying a sodium translocating ATPase. The medium including less than about 0.01 grams per liter yeast extract, less than about 0.01 grams per liter carbohydrate, a sodium ion concentration provided by a sodium ion feed rate of about 290 to about 8750 μg/gram of cells/minute, and a pH of about 4 to about 6.9.

The present invention provides a method of enhancing continuous directional high-value biological conversion of an urban wet garbage open system. The method includes wet garbage crushing, low-energy consumption hydrolysis, continuous conversion of organic components of wet garbage into short-chain fatty acid, continuous directional conversion of other components of short-chain fatty acid into acetic acid, separation and microbial reflux of acetic acid, and the like. In this method, by crushing wet garbage, performing low-energy consumption hydrolysis, and seeding acclimatized activated sludge, two stages of anaerobic fermentations are carried out to firstly convert organic components of the wet garbage continuously into short-chain fatty acid, and then continuously and directionally convert other components of short-chain fatty acid into acetic acid, so as to realize continuous directional high-value biological conversion of the urban wet garbage in an open system without adding pure microbes and a large amount of chemicals.

The present invention provides a method of enhancing continuous directional high-value biological conversion of an urban wet garbage open system. The method includes wet garbage crushing, low-energy consumption hydrolysis, continuous conversion of organic components of wet garbage into short-chain fatty acid, continuous directional conversion of other components of short-chain fatty acid into acetic acid, separation and microbial reflux of acetic acid, and the like. In this method, by crushing wet garbage, performing low-energy consumption hydrolysis, and seeding acclimatized activated sludge, two stages of anaerobic fermentations are carried out to firstly convert organic components of the wet garbage continuously into short-chain fatty acid, and then continuously and directionally convert other components of short-chain fatty acid into acetic acid, so as to realize continuous directional high-value biological conversion of the urban wet garbage in an open system without adding pure microbes and a large amount of chemicals.