The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.

The present invention relates to a promoter system inducing expression of 3-hydroxypropionic acid (3-HP) and a method of biologically producing 3-HP using the same. To improve production of 3-HP in a biological manner, continuous synthesis of new enzymes having enzyme activity is necessary. As a result of screening 3-HP reactive transcription regulators and 3-HP reactive promoters from several microorganisms including Pseudomonas denitrificans, it was confirmed that the transcriptions regulations and promoters are composed of LysR proteins and particular gene nucleotide sequences binding to the LysR proteins. Therefore, the 3-HP inducible system is expected to be effectively used to regulate 3-HP metabolic pathways.

The present invention relates to a promoter system inducing expression of 3-hydroxypropionic acid (3-HP) and a method of biologically producing 3-HP using the same. To improve production of 3-HP in a biological manner, continuous synthesis of new enzymes having enzyme activity is necessary. As a result of screening 3-HP reactive transcription regulators and 3-HP reactive promoters from several microorganisms including Pseudomonas denitrificans, it was confirmed that the transcriptions regulations and promoters are composed of LysR proteins and particular gene nucleotide sequences binding to the LysR proteins. Therefore, the 3-HP inducible system is expected to be effectively used to regulate 3-HP metabolic pathways.

Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.

Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.

The disclosure provides -ketoisocaproic acid decarboxylase and -keto-3-methylvaleric acid decarboxylase and recombinant microorganisms that host these enzymes. Methods involve the use of recombinant microorganisms to increase the production of isoamyl alcohols, their corresponding acids and their derivatives from carbon sources.

The disclosure provides -ketoisocaproic acid decarboxylase and -keto-3-methylvaleric acid decarboxylase and recombinant microorganisms that host these enzymes. Methods involve the use of recombinant microorganisms to increase the production of isoamyl alcohols, their corresponding acids and their derivatives from carbon sources.

A CO.sub.2, bioconversion process includes providing a CO.sub.2 containing substrate to a bioreactor, the CO.sub.2 containing substrate including about 5 to about 90 mole % CO.sub.2; and fermenting the CO.sub.2 containing substrate with an acetogenic bacteria carrying a sodium translocating ATPase. The medium including less than about 0.01 grams per liter yeast extract, less than about 0.01 grams per liter carbohydrate, a sodium ion concentration provided by a sodium ion feed rate of about 290 to about 8750 g/gram of cells/minute, and a pH of about 4 to about 6.9.

A CO.sub.2, bioconversion process includes providing a CO.sub.2 containing substrate to a bioreactor, the CO.sub.2 containing substrate including about 5 to about 90 mole % CO.sub.2; and fermenting the CO.sub.2 containing substrate with an acetogenic bacteria carrying a sodium translocating ATPase. The medium including less than about 0.01 grams per liter yeast extract, less than about 0.01 grams per liter carbohydrate, a sodium ion concentration provided by a sodium ion feed rate of about 290 to about 8750 g/gram of cells/minute, and a pH of about 4 to about 6.9.

The patent application relates to a method of producing a monomer component from a genetically modified polyhydroxyalkanoate (PHA) biomass, wherein the biomass is heated in the presence of a catalyst to release a monomer component from the PHA.