Provided herein are systems and methods for recycling and supplementing off-gas from a gas fed reaction process. The systems and methods are particularly useful for bioprocesses that convert hydrogen gas into one or more biosynthetic products. By maintaining separate hydrogen and oxygen feed gas streams, and forming a recycle gas that introduces a target component of the supply gas to the bioreactor within a target concentration range, the yields, productivities, and safety profiles of the bioprocess can be enhanced.

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or other materials are processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems and methods are described that can be used to treat feedstock materials, such as cellulosic and/or lignocellulosic materials, in a vault in which the walls and optionally the ceiling include discrete units. Such vaults are re-configurable.

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or other materials are processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems and methods are described that can be used to treat feedstock materials, such as cellulosic and/or lignocellulosic materials, in a vault in which the walls and optionally the ceiling include discrete units. Such vaults are re-configurable.

A fermentation product manufacturing process includes fermenting under fermentation conditions in an aqueous fermentation medium in a fermentation reactor a carbohydrate source with a microorganism capable of converting the carbohydrate into a fermentation product which is a salt or a product with a boiling point above the boiling point of water, during the process withdrawing part of the medium including biomass from the reactor in the form of a recycle stream, providing the stream including biomass to a pressure vessel wherein the pressure is such that the temperature of the stream decreases 1-8 C., as compared to the temperature of the medium in the reactor, by the evaporation of water, and recycling the cooled recycle stream to the reactor. The process makes it possible to obtain a homogeneous temperature profile of the fermentation medium with limited occurrence of hot or cool spots within the reactor which results in improved fermentation performance.

The present invention relates to a process of recovering 3-hydroxypropionic acid, comprising: forming a 3-hydroxypropionate crystal in a concentrate containing 3-hydroxypropionic acid in the presence of an alkali metal salt, preparing a solution containing 3-hydroxypropionate crystal separated from the concentrate, stirring an acid and the solution containing 3-hydroxypropionate crystal to form a precipitate, and subjecting the precipitate to a first washing, and a slurry composition comprising a precipitate prepared in the process of recovering 3-hydroxypropionic acid and 3-hydroxypropionic acid.

The present invention relates to a process of recovering 3-hydroxypropionic acid, comprising: forming a 3-hydroxypropionate crystal in a concentrate containing 3-hydroxypropionic acid in the presence of an alkali metal salt, preparing a solution containing 3-hydroxypropionate crystal separated from the concentrate, stirring an acid and the solution containing 3-hydroxypropionate crystal to form a precipitate, and subjecting the precipitate to a first washing, and a slurry composition comprising a precipitate prepared in the process of recovering 3-hydroxypropionic acid and 3-hydroxypropionic acid.

The present invention provides a semi-conducting biogenic hybrid catalyst capable of reducing CO.sub.2 into fuel precursors. Specifically, the present application involves a method for bio-assisted conversion of CO.sub.2 to fuel precursors using said semiconducting biogenic hybrid catalyst in batch and continuous mode.

The present invention relates to a two-stage process for production of drop-in fuels/alcohols (methanol, ethanol or butanol) from volatile fatty acids produced either synthetically from fossil resources or as metabolic intermediates in acidification step of anaerobic digestion process from waste biomass and organic materials.

A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.