Described are compositions and methods relating to modified yeast that over-express the glycerol-deficient suppressor, GDS1. The yeast produces an increased amount of ethanol and a deceased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.

Provided is a method which enables efficient separation of a component such as microorganisms from an organic substance-containing liquid obtained by microbial fermentation. Disclosed is a method for producing an organic substance comprising a microbial fermentation step of obtaining an organic substance-containing liquid and a separation step of heating the organic substance-containing liquid and separating into a liquid or solid component containing microorganisms and a gaseous component containing the organic substance.

A Lactobacillus paracasei and uses thereof. The Lactobacillus paracasei has a deposit number of CGMCC No. 14813. The Lactobacillus paracasei can be used for increasing the amount of an organic acid in a raw material. The Lactobacillus paracasei can be used for fermenting the raw material, where the raw material can be selected from at least one of fruits, which can increase the content of the organic acid in a fermented product.

A Lactobacillus paracasei and uses thereof. The Lactobacillus paracasei has a deposit number of CGMCC No. 14813. The Lactobacillus paracasei can be used for increasing the amount of an organic acid in a raw material. The Lactobacillus paracasei can be used for fermenting the raw material, where the raw material can be selected from at least one of fruits, which can increase the content of the organic acid in a fermented product.

Provided herein are methods of organic waste stream conversion including fermenting an organic waste stream with an inoculum using arrested methanogenesis to generate an organic product. The inoculum includes an anaerobic consortium isolated from cheese, yogurt, saline soil, kefir, and/or probiotics and the anaerobic consortium is pretreated to transform the anaerobic consortium into an acidogenic consortium

Provided herein are methods of organic waste stream conversion including fermenting an organic waste stream with an inoculum using arrested methanogenesis to generate an organic product. The inoculum includes an anaerobic consortium isolated from cheese, yogurt, saline soil, kefir, and/or probiotics and the anaerobic consortium is pretreated to transform the anaerobic consortium into an acidogenic consortium

A method of producing a volatile chemical substance uses a culture apparatus including: a culture tank configured to culture a microorganism having an ability to produce a volatile chemical substance; a delivery line configured to deliver a culture liquid obtained by culture in the culture tank to the storage tank; and a storage tank configured to store a solution containing the chemical substance obtained by culturing the microorganism, the method including culturing the microorganism in the culture tank while retaining the solution in the storage tank, wherein a gas phase portion in the storage tank and a gas phase portion in the culture tank are connected to each other through a gas connection pipe.

A method of producing a volatile chemical substance uses a culture apparatus including: a culture tank configured to culture a microorganism having an ability to produce a volatile chemical substance; a delivery line configured to deliver a culture liquid obtained by culture in the culture tank to the storage tank; and a storage tank configured to store a solution containing the chemical substance obtained by culturing the microorganism, the method including culturing the microorganism in the culture tank while retaining the solution in the storage tank, wherein a gas phase portion in the storage tank and a gas phase portion in the culture tank are connected to each other through a gas connection pipe.

The present application discloses a method of culturing a Euglena sp. microorganism, Schizochytrium sp. microorganism, or a Chlorella sp. microorganism comprising culturing the microorganism in a hybrid culture media; maintaining the microorganism heterotrophically in an environment substantially free from light; wherein the hybrid culture media comprises a carbohydrate; and wherein the hybrid culture media comprises fresh media and recycled culture media. The present application also discloses a hybrid culture media.

The present application discloses a method of culturing a Euglena sp. microorganism, Schizochytrium sp. microorganism, or a Chlorella sp. microorganism comprising culturing the microorganism in a hybrid culture media; maintaining the microorganism heterotrophically in an environment substantially free from light; wherein the hybrid culture media comprises a carbohydrate; and wherein the hybrid culture media comprises fresh media and recycled culture media. The present application also discloses a hybrid culture media.