A method for producing a fermentate including the steps of obtaining a fruit or vegetable extract, treating the extract with a hydrolytic enzyme, mixing the extract with a fermenting microorganism, water, and a growth media to produce a liquid composition; and incubating the liquid composition at a controlled temperature and a controlled pH to produce a fermentate. A method for killing or inhibiting the growth of a contaminating microorganism on or within a food product, and a food product including a fermentate having a cellular mass component from a fermenting microorganism, a fermented fruit or vegetable extract and a hydrolytic enzyme are disclosed. A fermentate produced by any one of the methods described is contemplated. The fermentate may be a concentrated liquid or a dry powder and has the ability to inhibit the growth of a contaminating microorganism by 100% when diluted to less than 5% (w/v).

A system for the production of high value chemicals includes (a) an input selected from the group consisting of ethylene glycol, glycerol, ethanol methanol or a combination thereof. In addition, the system includes (b) an oxidation biocatalyst including an alcohol oxidase, a copper radical oxidase, a glycerol oxidase, an alditol oxidase or a combination thereof. Further, the system includes (c) an oxidized intermediate. The system also includes (d) a finishing catalyst including a supported metal catalyst, a carboligating catalyst, an amine oxidase, a glyoxalase, an acid catalyst, a base catalyst, an isomerization catalyst or a combination thereof. Still further, the system includes (e) an output.

A system for the production of high value chemicals includes (a) an input selected from the group consisting of ethylene glycol, glycerol, ethanol methanol or a combination thereof. In addition, the system includes (b) an oxidation biocatalyst including an alcohol oxidase, a copper radical oxidase, a glycerol oxidase, an alditol oxidase or a combination thereof. Further, the system includes (c) an oxidized intermediate. The system also includes (d) a finishing catalyst including a supported metal catalyst, a carboligating catalyst, an amine oxidase, a glyoxalase, an acid catalyst, a base catalyst, an isomerization catalyst or a combination thereof. Still further, the system includes (e) an output.

Industrial fermentation for the production of lactic acid from organic waste combined with chemical recycling of polylactic acid are provided, to obtain lactic acid at high yields.

Industrial fermentation for the production of lactic acid from organic waste combined with chemical recycling of polylactic acid are provided, to obtain lactic acid at high yields.

The present invention provides an acid-tolerant Saccharomyces cerevisiae strain and use thereof. By using exogenously added malic acid as a stress, an acid-tolerant mutant S. cerevisiae strain MTPfo-4 is obtained by directed evolution screening in the laboratory, which tolerates a minimum pH of 2.44. The mutant strain MTPfo-4, tolerant to multiple organic acids, has an increased tolerance to exogenous malic acid of up to 86.6 g/L. The mutant strain MTPfo-4 obtained is further identified. The mutant strain grows stably and well, and can tolerate a variety of organic acids (lactic acid, malic acid, succinic acid, fumaric acid, citric acid, gluconic acid, and tartaric acid). It also has a strong tolerance to inorganic acids (HCl and H.sub.3PO.sub.4). This is difficult to achieve in the existing research and reports of S. cerevisiae. The strain is intended to be used as an acid-tolerant chassis cell factory for producing various short-chain organic acids.

The present disclosure provides a Klebsiella pneumoniae Y7-3 with a deposit number of CCTCC NO: M2019851. The strain can degrade corn stover into acetic acid, ethanol, and hydrogen, and can further metabolize into acetic acid, ethanol, 1,3-propanediol, lactic acid, and hydrogen.

The present disclosure provides a Klebsiella pneumoniae Y7-3 with a deposit number of CCTCC NO: M2019851. The strain can degrade corn stover into acetic acid, ethanol, and hydrogen, and can further metabolize into acetic acid, ethanol, 1,3-propanediol, lactic acid, and hydrogen.

The present invention provides a fermented honey product obtained by fermenting a honey-containing material having a honey concentration of more than 25% with Lactobacillus kunkeei.

A process for converting magnesium chloride into magnesium oxide having the steps of: subjecting a magnesium chloride solution to a spray drying step in a spray-drying apparatus at a temperature of 300-475° C., resulting in the formation of a spray-dried product having 10-80 wt. % magnesium oxide and 20-90 wt. % of the total of magnesium hydroxychloride and magnesium chloride, subjecting the product of the spray drying step to a roasting step in a roaster at a temperature of 600-900° C. in the presence of water, resulting in the formation of a product having at least 98 wt. % of MgO, and less than 2 wt. % of the total of magnesium hydroxychloride and magnesium chloride, wherein the percentages of MgO, magnesium hydroxychloride and magnesium chloride, are calculated on the total of MgO, magnesium hydroxychloride and magnesium chloride.