The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid.

The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid.

The present invention discloses an engineering yeast strain for producing nervonic acids. The yeast strain over-expresses the genes related to enzymes required in a synthetic process of long-chain unsaturated fatty acids, such as fatty acid elongase, desaturase, diacylglycerol acyltransferase and the like, and optionally, further adjusts and controls the synthesis and decomposition route of triglyceride, the synthesis and decomposition route of sphingomyelin, and the synthesis and decomposition route and the oxidation-reduction balanced route of lipid subcell levels. The recombinant yeast strain can produce microorganism oil; and the content of the prepared nervonic acids accounts for 39.6% of the total fatty acids.

The present invention discloses an engineering yeast strain for producing nervonic acids. The yeast strain over-expresses the genes related to enzymes required in a synthetic process of long-chain unsaturated fatty acids, such as fatty acid elongase, desaturase, diacylglycerol acyltransferase and the like, and optionally, further adjusts and controls the synthesis and decomposition route of triglyceride, the synthesis and decomposition route of sphingomyelin, and the synthesis and decomposition route and the oxidation-reduction balanced route of lipid subcell levels. The recombinant yeast strain can produce microorganism oil; and the content of the prepared nervonic acids accounts for 39.6% of the total fatty acids.

The object of an aspect of the present invention is to provide a method for producing a long-chain fatty acid, the method making it possible to prevent a coloring component from being contained in the long-chain fatty acid and recover the long-chain fatty acid at a high yield. The above problem is solved by providing a method for producing a long-chain fatty acid, the method including the steps of: (a) with use of an enzyme, decomposing, into fatty acids, triglyceride contained in a raw vegetable oil which is derived from a vegetable-derived oil-containing waste; (b) removing, by distillation, a short-chain fatty acid contained in the raw vegetable oil which has been subjected to the step (a); and (c) recovering, by short-path distillation, a long-chain fatty acid contained in the raw vegetable oil which has been subjected to the step (b).

The object of an aspect of the present invention is to provide a method for producing a long-chain fatty acid, the method making it possible to prevent a coloring component from being contained in the long-chain fatty acid and recover the long-chain fatty acid at a high yield. The above problem is solved by providing a method for producing a long-chain fatty acid, the method including the steps of: (a) with use of an enzyme, decomposing, into fatty acids, triglyceride contained in a raw vegetable oil which is derived from a vegetable-derived oil-containing waste; (b) removing, by distillation, a short-chain fatty acid contained in the raw vegetable oil which has been subjected to the step (a); and (c) recovering, by short-path distillation, a long-chain fatty acid contained in the raw vegetable oil which has been subjected to the step (b).

Systems, methods, and host cells utilizing a PopQC construct for enhancing product biosynthesis by exploitation of non-genetic cell-to-cell variation are disclosed. The PopQC construct includes at least a product-responsive biosensor and a selection gene.

Systems, methods, and host cells utilizing a PopQC construct for enhancing product biosynthesis by exploitation of non-genetic cell-to-cell variation are disclosed. The PopQC construct includes at least a product-responsive biosensor and a selection gene.

The application generally relates to bioproduction of molecules of interest in microorganisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to express or overexpress a mutant of a bubblegum-type acyl-CoA synthase, and uses thereof.

The application generally relates to bioproduction of molecules of interest in microorganisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to express or overexpress a mutant of a bubblegum-type acyl-CoA synthase, and uses thereof.