Patent classifications
C12P13/14
Microorganism having carbon dioxide fixation pathway introduced thereinto
- Ryota Fujii ,
- Tomokazu Shirai ,
- Tadashi Araki ,
- Koh Amano ,
- Yoshiko Matsumoto ,
- Toshihiro Tateno ,
- Nozomi Takebayashi ,
- Takashi Morishige ,
- Hitoshi Takahashi ,
- Mitsufumi Wada ,
- Hiroshi Shimizu ,
- Chikara Furusawa ,
- Takashi Hirasawa ,
- Tomonori Hidesaki ,
- Ayako Endo ,
- Dominik Lukas Jürgen-Lohmann ,
- Anjali Madhavan ,
- Su Sun Chong
An acetyl-CoA-producing microorganism, which is capable of efficiently synthesizing acetyl-CoA using carbon dioxide, and a substance production method using the same are provided. An acetyl-CoA-producing microorganism including an acetyl-CoA production cycle obtained by imparting at least one type of enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism.
MICROORGANISM FOR PRODUCING L-GLUTAMINE AND METHOD FOR PRODUCING L-GLUTAMINE USING SAME
Provided are a Corynebacterium glutamicum mutant that is resistant to high concentrations of L-glutamine, and a method of producing L-glutamine by using the mutant.
MICROORGANISM FOR PRODUCING L-GLUTAMINE AND METHOD FOR PRODUCING L-GLUTAMINE USING SAME
Provided are a Corynebacterium glutamicum mutant that is resistant to high concentrations of L-glutamine, and a method of producing L-glutamine by using the mutant.
MUTANT STRAIN HAVING ENHANCED L-GLUTAMIC ACID PRODUCING ABILITY, AND L-GLUTAMIC ACID PREPARATION METHOD USING SAME
The present disclosure relates to a mutant strain having enhanced L-glutamic acid productivity and a method of producing L-glutamic acid using the same. The mutant strain according to one embodiment of the present disclosure has reduced production of citramalate as a by-product due to weakening or inactivation of the activity of citramalate synthase and has excellent L-glutamic acid productivity. The strain having an additional mutation in the YggB protein may produce L-glutamic acid in an improved yield due to enhancement of glutamic acid release. Thus, when the mutant strain is used, it is possible to more effectively produce L-glutamic acid.
MUTANT STRAIN HAVING ENHANCED L-GLUTAMIC ACID PRODUCING ABILITY, AND L-GLUTAMIC ACID PREPARATION METHOD USING SAME
The present disclosure relates to a mutant strain having enhanced L-glutamic acid productivity and a method of producing L-glutamic acid using the same. The mutant strain according to one embodiment of the present disclosure has reduced production of citramalate as a by-product due to weakening or inactivation of the activity of citramalate synthase and has excellent L-glutamic acid productivity. The strain having an additional mutation in the YggB protein may produce L-glutamic acid in an improved yield due to enhancement of glutamic acid release. Thus, when the mutant strain is used, it is possible to more effectively produce L-glutamic acid.
Processing biomass
Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.
Processing biomass
Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.
METHOD FOR PRODUCING YEAST CONTAINING RICH-TASTE-IMPARTING SUBSTANCE AND METHOD FOR PRODUCING YEAST EXTRACT CONTAINING RICH-TASTE-IMPARTING SUBSTANCE
A method for producing a rich taste imparting substance-containing yeast, where the method includes: a yeast proliferating step of culturing a yeast that is modified to have a reduced acetolactate synthase activity in cells, and has isoleucine and valine requirements in a culture medium containing isoleucine and valine, to proliferate the yeast; and a rich taste imparting substance producing step of culturing the yeast with addition of valine to the culture medium when an isoleucine content in the culture medium is less than 0.2% by mass, to produce a rich taste imparting substance, wherein the rich taste imparting substance is at least one of γ-Glu-Abu and γ-Glu-Abu-Gly.
METHOD FOR PRODUCING YEAST CONTAINING RICH-TASTE-IMPARTING SUBSTANCE AND METHOD FOR PRODUCING YEAST EXTRACT CONTAINING RICH-TASTE-IMPARTING SUBSTANCE
A method for producing a rich taste imparting substance-containing yeast, where the method includes: a yeast proliferating step of culturing a yeast that is modified to have a reduced acetolactate synthase activity in cells, and has isoleucine and valine requirements in a culture medium containing isoleucine and valine, to proliferate the yeast; and a rich taste imparting substance producing step of culturing the yeast with addition of valine to the culture medium when an isoleucine content in the culture medium is less than 0.2% by mass, to produce a rich taste imparting substance, wherein the rich taste imparting substance is at least one of γ-Glu-Abu and γ-Glu-Abu-Gly.
APPLICATION OF TREHALASE IN FERMENTATIVE PRODUCTION
Provided is an application of trehalase in fermentative production. The trehalase has amino acid sequences shown in SEQ ID NO.6, SEQ ID NO.7, and SEQ ID NO.8. Provided are methods for producing and applying trehalase, particularly being applied in the production and fermentation of alcohol and an amino acid.