The present disclosure relates to a protein variant having a tryptophan-exporting activity, an L-tryptophan-producing microorganism expressing the protein variant, and a method for producing L-tryptophan using the microorganism.

This disclosure relates to modified tryptophan synthase and more particularly to modified beta-subunits of tryptophan synthase. The disclosure further relates to cells expressing such modified subunits and methods of producing non-canonical amino acids.

The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity and enhanced acid stability, while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.

Provided herein, inter alia, are genetically engineered microbes and methods of use thereof for producing L-4-chlorokynrenine from L-Tryptophan. The genetically engineered microbes include, inter alia, one or more exogenous nucleic acids or enzymes for producing L-4-chlorokynrenine.

Provided herein, inter alia, are genetically engineered microbes and methods of use thereof for producing L-4-chlorokynrenine from L-Tryptophan. The genetically engineered microbes include, inter alia, one or more exogenous nucleic acids or enzymes for producing L-4-chlorokynrenine.

The present invention generally relates to the microbiological industry, and specifically to the production of L-serine or L-serine derivatives using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms.

The present invention generally relates to the microbiological industry, and specifically to the production of L-serine or L-serine derivatives using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms.

The present invention concerns proteins having improved omega-transaminase (ω-TA) activity, nucleic acid molecules encoding respective proteins having improved ω-TA activity and methods for stereo selective synthesis of chiral amines and amino acids or increasing of chiral amines isomers in enantiomer mixtures.

The present invention concerns proteins having improved omega-transaminase (ω-TA) activity, nucleic acid molecules encoding respective proteins having improved ω-TA activity and methods for stereo selective synthesis of chiral amines and amino acids or increasing of chiral amines isomers in enantiomer mixtures.

Disclosed herein are recombinant host cells and methods capable of producing a tyramine containing hydroxycinnamic acid amide compounds, derivatives and extracts. Some embodiments provided include, for example, consumable products containing the tyramine containing hydroxycinnamic acid amide produced by the recombinant host cells. Some embodiments provided herein are methods for producing a tyramine containing hydroxycinnamic acid amide or hydroxycinnamic acid amide derivative.