The present disclosure provides an isolated strain of Amycolatopsis sp. WGS_07 having accession number MCC 0218. The present disclosure provides a process for synthesizing furano-naphthoquinone and pyrazinone derivatives using a novel strain Amycolatopsis sp. . The novel compounds synthesized possesses activity against gram positive bacteria, malarial parasites, and various cancers.

A method for increasing the molecular diversity of polyketides and non-ribsomomal peptides by using recombination to efficiently increase or decrease the number of modules in the polyketide synthase or non-ribosomal peptide synthetase encoding said polyketide or peptide.

The present invention relates to culture conditions for high production of the antibiotic aureothin from Streptomyces thioluteus.

Provided is a method for preparing a theaflavin using an immobilized substrate, including: enriching a catechin molecule substrate, a natural plant monomer, on an inert adsorption carrier in a non-covalent bonding manner; subjecting a resulting material to purification; and catalyzing polymerization of the catechin molecule substrate enriched on the carrier with a high-selectivity free exogenous enzyme to produce the dimeric substance of theaflavin. The purification and enzymatic oxidation condensation of the catechin substrate are integrated on a same carrier, which reduces the difficulty of process operations.

Provided are a glycosyltransferase UGT76G1 mutant, an isolated polynucleotide, a carrier, a production method, and a composition and a kit for the glycosylation of substrates. Further provided are a method for increasing the catalytic activity of the glycosyltransferase UGT76G1 mutant, and a method for promoting the glycosylation of flavone compounds and the use thereof. The glycosyltransferase UGT76G1 mutant is subjected to mutations at positions corresponding to positions 87, 199, 204 or 379 of SEQ ID NO: 1, and significantly improves the transformation activity of substrates of the flavone compounds.

Provided is a method for preparing a theaflavin using an immobilized substrate, including: enriching a catechin molecule substrate, a natural plant monomer, on an inert adsorption carrier in a non-covalent bonding manner; subjecting a resulting material to purification; and catalyzing polymerization of the catechin molecule substrate enriched on the carrier with a high-selectivity free exogenous enzyme to produce the dimeric substance of theaflavin. The purification and enzymatic oxidation condensation of the catechin substrate are integrated on a same carrier, which reduces the difficulty of process operations.

A method for producing a cannabinoid by contacting cannabigerolic acid with a cannabinoid synthase orthologue. The cannabinoid synthase orthologue is from an organism other than Cannabis sativa. Also disclosed is a recombinant cell of Saccharomyces cerevisiae or Pichia pastoris that includes in its genome a nucleic acid encoding the above cannabinoid synthase orthologue. The cannabinoid synthase orthologue is expressed in the recombinant cell in an active form.