Techniques regarding screening for mutations using nanoscale deterministic arrays are provided. For example, one or more embodiments described herein can comprise a method, which can comprise cleaving a deoxyribonucleic acid segment hybridized with a molecular probe to form a sample fluid. The cleaving can occur at a first end and a second end of the molecular probe. Also, the cleaving can comprise a cleaving agent that targets base pair mismatches. The method can also comprise supplying the sample fluid to a nanoscale deterministic lateral displacement array to screen for a single nucleotide polymorphism.

Methods and apparatuses are disclosed for detecting a presence of a mismatched pair in an oligonucleotide duplex that is attached to a solid substrate using an atomic force microscope. In particular, methods and apparatuses of the invention allow qualitative and quantitative analysis of the presence of a mismatched pair in a sample of oligonucleotide duplex using an atomic force microscope comprising an AFM cantilever that includes a DNA mismatch repair protein. Methods and apparatuses of the invention allow detection of gene mutation without a need for amplification, labeling, or modification of the sample. Such apparatuses and methods can be used in a wide variety of clinical diagnostic applications including detection and/or analysis of biomarkers related to, but not limited to, cancer, trauma, sepsis, aseptic inflammation, myocardial infarction, stroke, transplantation, diabetes, sickle cell disease, as well as other clinical conditions.

Methods and apparatuses are disclosed for detecting a presence of a mismatched pair in an oligonucleotide duplex that is attached to a solid substrate using an atomic force microscope. In particular, methods and apparatuses of the invention allow qualitative and quantitative analysis of the presence of a mismatched pair in a sample of oligonucleotide duplex using an atomic force microscope comprising an AFM cantilever that includes a DNA mismatch repair protein. Methods and apparatuses of the invention allow detection of gene mutation without a need for amplification, labeling, or modification of the sample. Such apparatuses and methods can be used in a wide variety of clinical diagnostic applications including detection and/or analysis of biomarkers related to, but not limited to, cancer, trauma, sepsis, aseptic inflammation, myocardial infarction, stroke, transplantation, diabetes, sickle cell disease, as well as other clinical conditions.

Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.

Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.

Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.

Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.

Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.

Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.

Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.