The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, utilize a of cyclic and/or non-cyclic solvent that is non-toxic and may eliminate or reduce the amount of formamide in the hybridization composition.

This disclosure relates to a method for increasing the hybridization efficiency of a probe and a target RNA in a sample, for example to identify a particular RNA present in the sample. The method includes heating a lysate sample comprising at least one target RNA, such as a tRNA, mRNA or rRNA, at a temperature of about 95 C. for a time sufficient to interfere with secondary structure of the RNA, wherein the time is short enough, such that the RNA in the cell lysate sample are not significantly degraded, and wherein the lysate comprises a cell lysis buffer comprising a chemical denaturant. To detect a target RNA in the lysate, the lysate is contacted with at least one detectable probe, such as a labeled probe, designed to specifically hybridize to the target RNA in the lysate.

This disclosure relates to a method for increasing the hybridization efficiency of a probe and a target RNA in a sample, for example to identify a particular RNA present in the sample. The method includes heating a lysate sample comprising at least one target RNA, such as a tRNA, mRNA or rRNA, at a temperature of about 95 C. for a time sufficient to interfere with secondary structure of the RNA, wherein the time is short enough, such that the RNA in the cell lysate sample are not significantly degraded, and wherein the lysate comprises a cell lysis buffer comprising a chemical denaturant. To detect a target RNA in the lysate, the lysate is contacted with at least one detectable probe, such as a labeled probe, designed to specifically hybridize to the target RNA in the lysate.

Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.

Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.

DNA and RNA fluorescence in situ hybridization (FISH) is a widely used method to analyze the copy number and spatial localization of specific DNA and RNA sequences in the nuclear. Here we introduce an approach to achieve highly multiplexed sequential DNA or RNA FISH at a fast speed. In one approach, one first performs one-color live imaging using the CRISPR imaging method for multiple genomic loci and then uses sequential rounds of DNA FISH to determine the loci identity. The FISH protocol described herein has been developed so that each round of hybridization is complete in 1 min. for example demonstrating the identification of 7 genomic elements and the capability to sustain reversible staining and washing for example for up to 20 rounds. In another approach, one can profile the gene expression pattern of a single cell using rapid and sequential RNA FISH. Each round of RNA FISH is complete in 5-10 min.

DNA and RNA fluorescence in situ hybridization (FISH) is a widely used method to analyze the copy number and spatial localization of specific DNA and RNA sequences in the nuclear. Here we introduce an approach to achieve highly multiplexed sequential DNA or RNA FISH at a fast speed. In one approach, one first performs one-color live imaging using the CRISPR imaging method for multiple genomic loci and then uses sequential rounds of DNA FISH to determine the loci identity. The FISH protocol described herein has been developed so that each round of hybridization is complete in 1 min. for example demonstrating the identification of 7 genomic elements and the capability to sustain reversible staining and washing for example for up to 20 rounds. In another approach, one can profile the gene expression pattern of a single cell using rapid and sequential RNA FISH. Each round of RNA FISH is complete in 5-10 min.

The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.

The invention provides methods and compositions for hybridizing at least one molecule to a target. The composition comprises at least one nucleic acid sequence, formamide, and a hybridization solution, wherein the concentration of formamide is less than or equal to 25%.

The invention provides methods and compositions for hybridizing at least one molecule to a target. The composition comprises at least one nucleic acid sequence, formamide, and a hybridization solution, wherein the concentration of formamide is less than or equal to 25%.