The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.

A decision-making support method is presented for issuing warnings and selecting mitigation actions parameterized by the turn of weather and/or climate information into a single decision index. A decision-making support method was developed from the Global Weather Decision Index (WDI) or Climate Decision Index (CDI), which is based on user preferences in relation to three characteristics of weather-climate information: a) value of the weather-climate variable; b) probability of occurrence; and c) lead-time of weather-climate information. The presented embodiments were initially developed having as the field of application the area of aerospace meteorology, as motivation the rockets launch operations in space centers. However, the decision-making process under weather uncertainty is relevant in other applications where weather or climate conditions may cause some kind of impact on activities.

A decision-making support method is presented for issuing warnings and selecting mitigation actions parameterized by the turn of weather and/or climate information into a single decision index. A decision-making support method was developed from the Global Weather Decision Index (WDI) or Climate Decision Index (CDI), which is based on user preferences in relation to three characteristics of weather-climate information: a) value of the weather-climate variable; b) probability of occurrence; and c) lead-time of weather-climate information. The presented embodiments were initially developed having as the field of application the area of aerospace meteorology, as motivation the rockets launch operations in space centers. However, the decision-making process under weather uncertainty is relevant in other applications where weather or climate conditions may cause some kind of impact on activities.

Presented herein are methods and compositions for enhancing utilization of surface primers during the surface amplification process. The methods are useful for surface amplification at improved densities. The methods and compositions provided herein enable creation of clusters which are brighter, but at the same densities as currently achieved using standard cluster amplification.

Presented herein are methods and compositions for enhancing utilization of surface primers during the surface amplification process. The methods are useful for surface amplification at improved densities. The methods and compositions provided herein enable creation of clusters which are brighter, but at the same densities as currently achieved using standard cluster amplification.

The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells.

The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells.

Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.

Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.

The present disclosure provides methods and systems for analysis of an analyte. Analysis of an analyte may include contacting an analyte with a binding reagent, which binding reagent may include a binding probe with specificity for the analyte and a nucleic acid molecule. The nucleic acid molecule may be denatured and signals indicative of the denaturing of the analyte may be collected. The collected signals may be used to detect, identify, or quantify the analyte.