A method of immobilizing an oligonucleotide on a polypropylene surface involves oxidizing at least a portion of the polypropylene surface, and contacting an amine-terminated oligonucleotide with the oxidized polypropylene surface to immobilize the oligonucleotide to the oxidized polypropylene surface. The method of immobilizing labelled ssDNA on a polypropylene surface is useful in a hybridization assay.

A method of immobilizing an oligonucleotide on a polypropylene surface involves oxidizing at least a portion of the polypropylene surface, and contacting an amine-terminated oligonucleotide with the oxidized polypropylene surface to immobilize the oligonucleotide to the oxidized polypropylene surface. The method of immobilizing labelled ssDNA on a polypropylene surface is useful in a hybridization assay.

Various aspects described herein relate to electrochemical devices, e.g., for separation of one or more biomolecules from a solution, and methods of using the same. Methods for using the electrochemical devices for biocatalysis are also described herein.

Various aspects described herein relate to electrochemical devices, e.g., for separation of one or more biomolecules from a solution, and methods of using the same. Methods for using the electrochemical devices for biocatalysis are also described herein.

Some embodiments presented in this disclosure concern an Automated Tissue Dissection (ATD) System. An ATD system is a one stop, and potentially low-cost, system to perform dissections on a substrate from pathologist digital mark or pen mark on the substrate using non-contact and/or mechanical method to extract a Formalin-Fixed Paraffin-Embedded (FFPE) tissue sample with: (a) only the ROI or ROIs as area to be saved; and (b) remove or decompose nucleic acid content in the region of no interest (RONI) and collect all tissue sample from a standard microscope substrate into a specific container.

Among other things, the present disclosure provides compositions and methods for analysis of cytosine modifications in nucleic acid sequences. In some embodiments, the present disclosure provides subtraction-free methods for detection of cytosine modifications in one or more nucleic acid sequences.

Among other things, the present disclosure provides compositions and methods for analysis of cytosine modifications in nucleic acid sequences. In some embodiments, the present disclosure provides subtraction-free methods for detection of cytosine modifications in one or more nucleic acid sequences.

This disclosure provides methods for cost-effective bisulfite-free identification in DNA, including whole genomic DNA, of the locations of one or more of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine. The methods described herein are based on the conversion of modified cytosine (5mC, 5hmC, 5fC, 5caC) to dihydrouracil (DHU), for example by TET-assisted pyridine borane treatment, followed by endonuclease cleavage of the DHU, and identification of the cleavage site, which corresponds to the location of the modified cytosine.

This disclosure provides methods for cost-effective bisulfite-free identification in DNA, including whole genomic DNA, of the locations of one or more of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine. The methods described herein are based on the conversion of modified cytosine (5mC, 5hmC, 5fC, 5caC) to dihydrouracil (DHU), for example by TET-assisted pyridine borane treatment, followed by endonuclease cleavage of the DHU, and identification of the cleavage site, which corresponds to the location of the modified cytosine.

Provided herein are methods, systems, and compositions for determining a base in a polynucleotide. In various aspects, the methods, systems, and compositions presented herein are useful for performing 4-base, 5-base, or 6-base sequencing of polynucleotide molecules, for example, from liquid biopsy samples or wherein the base is a low frequency mutation.