Systems and methods for light based heating of light absorbing sources for modification of nucleic acids through fast thermal cycling of polymerase chain reaction are described.

Systems and methods for light based heating of light absorbing sources for modification of nucleic acids through fast thermal cycling of polymerase chain reaction are described.

A micromechanical sensor comprising a nucleic acid or nucleic acid analog nanostructure, such as a DNA origami or tiled structure, sensor spring and fluorophores arranged in FRET pairs in the sensor spring is provided, as well as methods of using the micromechanical sensor.

Methods for preparing enriched sequencing libraries from test samples that contain double-stranded deoxyribonucleic acid (dsDNA) are provided.

Methods for preparing enriched sequencing libraries from test samples that contain double-stranded deoxyribonucleic acid (dsDNA) are provided.

The invention relates to a process for pre-concentration and detection of at least one single-stranded nucleic acid target molecule, the process comprising steps of generating a flow of liquid comprising at least one magnetic nanoparticle in a micro-channel and a plurality of single-stranded nucleic acid probe molecules attached to the nanoparticle, of generating an alternating magnetic field in the part of the micro-channel by means of the electromagnet, the magnetic field having an intensity and frequency that are suitable for causing magnetic hyperthermia of the nanoparticles so as to cause denaturing of the duplex formed by the single-stranded nucleic acid target molecule and the single-stranded nucleic acid probe molecule, and detection of the single-stranded target molecule dispersed in the liquid.

The invention relates to a process for pre-concentration and detection of at least one single-stranded nucleic acid target molecule, the process comprising steps of generating a flow of liquid comprising at least one magnetic nanoparticle in a micro-channel and a plurality of single-stranded nucleic acid probe molecules attached to the nanoparticle, of generating an alternating magnetic field in the part of the micro-channel by means of the electromagnet, the magnetic field having an intensity and frequency that are suitable for causing magnetic hyperthermia of the nanoparticles so as to cause denaturing of the duplex formed by the single-stranded nucleic acid target molecule and the single-stranded nucleic acid probe molecule, and detection of the single-stranded target molecule dispersed in the liquid.

The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.

The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.

Provided herein, in some embodiments, are methods, compositions and kits for large-scale production of long single-stranded DNA in solution.