The present invention provides a method for detecting a microorganism in a sample, the method comprising: a) filtering a sample through a filter to entrap any microorganisms present in the sample; b) treating the filter to release genomic material or DNA from the entrapped microorganisms; c) amplifying the genomic material or DNA released from the entrapped microorganisms; and d) identifying specific regions of the genomic material or DNA to determine the presence, identify the species or quantify the approximate number of any entrapped microorganisms.

The present invention provides a method for detecting a microorganism in a sample, the method comprising: a) filtering a sample through a filter to entrap any microorganisms present in the sample; b) treating the filter to release genomic material or DNA from the entrapped microorganisms; c) amplifying the genomic material or DNA released from the entrapped microorganisms; and d) identifying specific regions of the genomic material or DNA to determine the presence, identify the species or quantify the approximate number of any entrapped microorganisms.

Provided herein is a method and device for performing a homogeneous nucleic acid detection assay. The device can contain a pair of plates where one of the plates comprises (i) surface amplification surface; and (ii) target-specific nucleic acid probes that are immobilized on said amplification surface and that specifically binds to a part of the target nucleic acid; and the second plate comprises a sample contact area comprising a reagent storage site that comprises target-specific nucleic acid detection agents that specifically binds to another part of the target nucleic acid. In some embodiments, the device can be read without a washing unbound label from the surface of the device.

Provided herein is a method and device for performing a homogeneous nucleic acid detection assay. The device can contain a pair of plates where one of the plates comprises (i) surface amplification surface; and (ii) target-specific nucleic acid probes that are immobilized on said amplification surface and that specifically binds to a part of the target nucleic acid; and the second plate comprises a sample contact area comprising a reagent storage site that comprises target-specific nucleic acid detection agents that specifically binds to another part of the target nucleic acid. In some embodiments, the device can be read without a washing unbound label from the surface of the device.

To slow down the speed of a biopolymer passing through a nanopore during electrophoresis to such a speed that enables a monomer sequence analysis to be performed. A biopolymer analysis device includes two tanks 101a and 101b each capable of storing a solution containing a biopolymer and an electrolyte, a pair of electrodes 105a and 105b, a thin film 104 with a nanopore, and a three-dimensional structure 103 disposed on the thin film. The three-dimensional structure has a void that can store a solution, and the void forms a flow channel, the flow channel being adapted to allow the solution to pass therethrough from the nanopore to a portion above the three-dimensional structure, and having on its surface a functional group capable of adsorbing the biopolymer. Thus, when a voltage is applied, the three-dimensional structure is not re-dispersed in the solution at least in the range of a hemisphere having the nanopore as the center and having a biopolymer trapping length r as the radius.

To slow down the speed of a biopolymer passing through a nanopore during electrophoresis to such a speed that enables a monomer sequence analysis to be performed. A biopolymer analysis device includes two tanks 101a and 101b each capable of storing a solution containing a biopolymer and an electrolyte, a pair of electrodes 105a and 105b, a thin film 104 with a nanopore, and a three-dimensional structure 103 disposed on the thin film. The three-dimensional structure has a void that can store a solution, and the void forms a flow channel, the flow channel being adapted to allow the solution to pass therethrough from the nanopore to a portion above the three-dimensional structure, and having on its surface a functional group capable of adsorbing the biopolymer. Thus, when a voltage is applied, the three-dimensional structure is not re-dispersed in the solution at least in the range of a hemisphere having the nanopore as the center and having a biopolymer trapping length r as the radius.

Methods for single-molecule analysis of structure and sequence of linearized polynucleotides are provided.

Methods for single-molecule analysis of structure and sequence of linearized polynucleotides are provided.

Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.