Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

Provided herein is a method for isolating high molecular weight (HMW) DNA using beads that are at least 200 m in diameter that utilizes a device for retaining the beads and where the purified DNA eluant exits the device without shearing the HMW DNA. In some embodiments, the method comprises precipitating the DNA onto the beads, washing the beads in the device, and then eluting the DNA from the beads therein while substantially avoiding shear. Compositions and kits for practicing the method are also provided.

Provided herein is a method for isolating high molecular weight (HMW) DNA using beads that are at least 200 m in diameter that utilizes a device for retaining the beads and where the purified DNA eluant exits the device without shearing the HMW DNA. In some embodiments, the method comprises precipitating the DNA onto the beads, washing the beads in the device, and then eluting the DNA from the beads therein while substantially avoiding shear. Compositions and kits for practicing the method are also provided.

The present disclosure provides a nucleic acid homogenization method, and a kit and use thereof. The method including at least the following steps: respectively adding nucleic acid adsorption materials having the same nucleic acid saturation adsorption amount into a plurality of nucleic acid solutions, and the nucleic acid adsorption materials added into each nucleic acid solution can all achieve a nucleic acid adsorption saturation state; separating the nucleic acid adsorption materials of the saturation absorbed nucleic acids; eluting the nucleic acids from the separated nucleic acid adsorption materials. The nucleic acid homogenization method disclosed in the present disclosure is easy to operate, and allows for rapid and stable equal proportional dilution of a nucleic acid, a PCR product or a high throughput sequencing library concentration.

The present disclosure provides a nucleic acid homogenization method, and a kit and use thereof. The method including at least the following steps: respectively adding nucleic acid adsorption materials having the same nucleic acid saturation adsorption amount into a plurality of nucleic acid solutions, and the nucleic acid adsorption materials added into each nucleic acid solution can all achieve a nucleic acid adsorption saturation state; separating the nucleic acid adsorption materials of the saturation absorbed nucleic acids; eluting the nucleic acids from the separated nucleic acid adsorption materials. The nucleic acid homogenization method disclosed in the present disclosure is easy to operate, and allows for rapid and stable equal proportional dilution of a nucleic acid, a PCR product or a high throughput sequencing library concentration.

In a method for the desorption of nucleic acids from a sample, in order to simplify the desorption of nucleic acids from the sample, a solid phase is repeatedly rinsed with an elution buffer in a microfluidic system, in order to elute nucleic acids bonded to the solid phase from the solid phase in the microfluidic system.

In a method for the desorption of nucleic acids from a sample, in order to simplify the desorption of nucleic acids from the sample, a solid phase is repeatedly rinsed with an elution buffer in a microfluidic system, in order to elute nucleic acids bonded to the solid phase from the solid phase in the microfluidic system.

A method for rapidly isolating a biological molecule for a nucleic acid amplification reaction from a biological sample, the method comprising: putting a cubical shaped-porous solid phase having a plurality of pores varied in size in contact with a biological sample to get the biological molecule present in the biological sample sucked into pores of the cubical shaped-porous solid phase, wherein the cubical shaped-porous solid phase is made of ceramic having oxide material, which is selected from the group consisting of Al2O3, Fe2O3, low temperature co-fired ceramic (LTCC), PbO, and ZnO.

A method for rapidly isolating a biological molecule for a nucleic acid amplification reaction from a biological sample, the method comprising: putting a cubical shaped-porous solid phase having a plurality of pores varied in size in contact with a biological sample to get the biological molecule present in the biological sample sucked into pores of the cubical shaped-porous solid phase, wherein the cubical shaped-porous solid phase is made of ceramic having oxide material, which is selected from the group consisting of Al2O3, Fe2O3, low temperature co-fired ceramic (LTCC), PbO, and ZnO.

A method of marking an inventory item includes providing an activatable smoke generator and a reservoir for holding a smoke fluid and adapted to provide a flow of smoke fluid to the generator. The reservoir contains a smoke fluid including a carrier nucleic acid having a uniquely identifiable sequence, and upon activation of the smoke generator, marker smoke is generated and targeted to flow over the inventory item. The method further includes activating the smoke generator to produce the marker smoke including the carrier nucleic acid so as to cause the marker smoke to flow over the inventory item and thereby to detectably mark the inventory item with carrier nucleic acid. The invention provides methods for stably binding and immobilizing deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate. The alkaline conditions are produced by mixing the deoxyribonucleic acid with an alkaline solution having a pH of about 9.0 or higher, and contacting the deoxyribonucleic acid to the substrate. The immobilized DNA can be used as a taggant and can be used in combination with other detectable taggants, such as optical reporters.