Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.

Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.

This disclosure provides methods and compositions for long fragment read sequencing. Technology is described for preparing long fragments of genomic DNA, for processing genomic DNA for long fragment read sequencing methods, as well as software and algorithms for processing and analyzing sequence data. Combinatorial oligonucleotide bar codes are used to label fragments from nearby portions of the genome, which facilitate computational assembly of sequence reads to obtain the genome sequence. This improves efficiency and accuracy of sequencing, whereby an entire sequence can be obtained from fragments that constitute a lower coverage amount of the genome.

This disclosure provides methods and compositions for long fragment read sequencing. Technology is described for preparing long fragments of genomic DNA, for processing genomic DNA for long fragment read sequencing methods, as well as software and algorithms for processing and analyzing sequence data. Combinatorial oligonucleotide bar codes are used to label fragments from nearby portions of the genome, which facilitate computational assembly of sequence reads to obtain the genome sequence. This improves efficiency and accuracy of sequencing, whereby an entire sequence can be obtained from fragments that constitute a lower coverage amount of the genome.

Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

The present invention relates to a method of detecting and quantifying biomolecules using nucleic acid polymerase activity controlled by the target molecule, and more particularly to a method for detecting or quantifying biomolecules, which can detect and quantify nucleic acids, proteins, small-molecular substances, physiologically active substances (enzymatic activities), etc., with high sensitivity, based on the change in DNA polymerase activity caused by specific binding of a specific nucleic acid that forms a complex with a DNA aptamer prepared so as to comprise a single-stranded DNA that specifically recognizes the specific nucleic acid. The present invention can provide a method for diagnosing biomolecules, which can detect and quantify target nucleic acids, target proteins, target small-molecular substances, target enzyme activities and the like in a label-free and sensitive manner by controlling polymerase activity through target molecule-induced conformational change of a DNA aptamer.

The present invention relates to a method of detecting and quantifying biomolecules using nucleic acid polymerase activity controlled by the target molecule, and more particularly to a method for detecting or quantifying biomolecules, which can detect and quantify nucleic acids, proteins, small-molecular substances, physiologically active substances (enzymatic activities), etc., with high sensitivity, based on the change in DNA polymerase activity caused by specific binding of a specific nucleic acid that forms a complex with a DNA aptamer prepared so as to comprise a single-stranded DNA that specifically recognizes the specific nucleic acid. The present invention can provide a method for diagnosing biomolecules, which can detect and quantify target nucleic acids, target proteins, target small-molecular substances, target enzyme activities and the like in a label-free and sensitive manner by controlling polymerase activity through target molecule-induced conformational change of a DNA aptamer.

The present invention relates to a method for introducing mutations into at least one target nucleic acid molecule comprising (a) providing at least one sample comprising at least one target nucleic acid molecule; and (b) amplifying the at least one target nucleic acid molecule using a low bias DNA polymerase. The present further relates to a use of a low bias DNA polymerase in a method for introducing mutations into one or more nucleic acid molecule(s), a group of sample tags, a method for designing the group of sample tags, a computer readable medium, and a method for preferentially amplifying target nucleic acid molecules.

The present invention relates to a method for introducing mutations into at least one target nucleic acid molecule comprising (a) providing at least one sample comprising at least one target nucleic acid molecule; and (b) amplifying the at least one target nucleic acid molecule using a low bias DNA polymerase. The present further relates to a use of a low bias DNA polymerase in a method for introducing mutations into one or more nucleic acid molecule(s), a group of sample tags, a method for designing the group of sample tags, a computer readable medium, and a method for preferentially amplifying target nucleic acid molecules.