Compounds useful as nucleic acid probes are disclosed. In some embodiments, the compounds have the following structure (I) or a stereoisomer, tautomer or salt thereof, wherein M, L.sup.1a, L.sup.2, L.sup.8, L.sup.1b, L.sup.3, L.sup.5, L.sup.6, L.sup.7, L.sup.4, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sub.a, R.sub.b, R.sub.c, R.sub.d, m, n, q, and w are as defined herein. Methods associated with preparation and use of such compounds are also provided.

Compounds useful as nucleic acid probes are disclosed. In some embodiments, the compounds have the following structure (I) or a stereoisomer, tautomer or salt thereof, wherein M, L.sup.1a, L.sup.2, L.sup.8, L.sup.1b, L.sup.3, L.sup.5, L.sup.6, L.sup.7, L.sup.4, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sub.a, R.sub.b, R.sub.c, R.sub.d, m, n, q, and w are as defined herein. Methods associated with preparation and use of such compounds are also provided.

The present invention is concerned with linear double stranded DNA, which is coupled to a single support or a tag at the 3′ end of its non-coding strand and methods for producing said linear double stranded DNA. The present invention further relates to the use of said linear double stranded DNA in an RNA in vitro transcription reaction and also to a method for producing RNA in vitro. The present invention also relates to a bioreactor for RNA in vitro transcription.

The present invention is concerned with linear double stranded DNA, which is coupled to a single support or a tag at the 3′ end of its non-coding strand and methods for producing said linear double stranded DNA. The present invention further relates to the use of said linear double stranded DNA in an RNA in vitro transcription reaction and also to a method for producing RNA in vitro. The present invention also relates to a bioreactor for RNA in vitro transcription.

The present disclosure provides a method for methylation analysis of genomic fragments.

The present disclosure provides a method for methylation analysis of genomic fragments.

A method for purifying nucleotides is provided, that includes preparing a solution comprising (a) 3′-blocked nucleotides, (b) 3′-OH nucleotides, (c) a polishing polymerase, and (d) a template. The polishing polymerase and the template are used to selectively polymerize the 3′-OH nucleotides and thus reduce a concentration in the solution of the 3′-OH nucleotides relative to the 3′-blocked nucleotides.

A method for purifying nucleotides is provided, that includes preparing a solution comprising (a) 3′-blocked nucleotides, (b) 3′-OH nucleotides, (c) a polishing polymerase, and (d) a template. The polishing polymerase and the template are used to selectively polymerize the 3′-OH nucleotides and thus reduce a concentration in the solution of the 3′-OH nucleotides relative to the 3′-blocked nucleotides.

Nucleoside or nucleotide analog compounds having the structure shown below, a method for preparing them, and applications in nucleic acid sequencing are disclosed. The compounds have formula (I): ##STR00001## wherein L.sub.1, L.sub.2, and L.sub.3 are each independently a covalent bond or a covalently linked group; B is a base or a base derivative selected from purines, pyrimidines, or analogs thereof; R.sub.1 is —OH, a phosphate group, or a nucleotide; R.sub.2 as H or a cleavable group; R.sub.3 is a detectable group or a targeting group; R.sub.5 is an inhibitory group; R.sub.4 is H, —NH.sub.2, or —OR.sub.6, wherein R.sub.6 is H or a cleavable group; and C is a cleavable group or a cleavable bond.

Nucleoside or nucleotide analog compounds having the structure shown below, a method for preparing them, and applications in nucleic acid sequencing are disclosed. The compounds have formula (I): ##STR00001## wherein L.sub.1, L.sub.2, and L.sub.3 are each independently a covalent bond or a covalently linked group; B is a base or a base derivative selected from purines, pyrimidines, or analogs thereof; R.sub.1 is —OH, a phosphate group, or a nucleotide; R.sub.2 as H or a cleavable group; R.sub.3 is a detectable group or a targeting group; R.sub.5 is an inhibitory group; R.sub.4 is H, —NH.sub.2, or —OR.sub.6, wherein R.sub.6 is H or a cleavable group; and C is a cleavable group or a cleavable bond.