A method of sample analysis is provided. In certain embodiments, the method may comprise: contacting a nucleic acid sample with a first primer and a second primer under PCR conditions to produce a double stranded product, wherein the second primer comprises a first label and is 5′ blocked; b) contacting the double stranded product with an exonuclease to degrade one strand of the double-stranded product to produce a single stranded product; c) contacting the single stranded product with a third primer under primer extension conditions, wherein the third primer comprises a second label; and d) detecting the first and second labels of the partial duplex. A kit for practicing the method is also provided.

A method of sample analysis is provided. In certain embodiments, the method may comprise: contacting a nucleic acid sample with a first primer and a second primer under PCR conditions to produce a double stranded product, wherein the second primer comprises a first label and is 5′ blocked; b) contacting the double stranded product with an exonuclease to degrade one strand of the double-stranded product to produce a single stranded product; c) contacting the single stranded product with a third primer under primer extension conditions, wherein the third primer comprises a second label; and d) detecting the first and second labels of the partial duplex. A kit for practicing the method is also provided.

Processes and kits for preparing a plurality of multiplex amplification products for targeted next generation-sequencing providing reduced background noise.

Processes and kits for preparing a plurality of multiplex amplification products for targeted next generation-sequencing providing reduced background noise.

A method of nucleic acid amplification involving using a first modified primer which provides protection to the amplification product from exonuclease degradation and a second primer. The method provides a double stranded nucleic acid, one strand of which is degraded by a double strand nucleic acid specific exonuclease to form a single stranded nucleic acid, which is protected from exonuclease degradation.

A method of nucleic acid amplification involving using a first modified primer which provides protection to the amplification product from exonuclease degradation and a second primer. The method provides a double stranded nucleic acid, one strand of which is degraded by a double strand nucleic acid specific exonuclease to form a single stranded nucleic acid, which is protected from exonuclease degradation.

The present disclosure is directed to methods and kits for detection and/or quantification of nucleic acids, such as double stranded DNA that is used in next-generation sequencing (NGS) applications. Nucleic acid-based probes, such as peptide nucleic acid (PNA) oligomers, molecular beacons, DNA flares and locked nucleic acid (LNA) oligomers, are also provided for use in the methods and kits of the present disclosure.

The present disclosure is directed to methods and kits for detection and/or quantification of nucleic acids, such as double stranded DNA that is used in next-generation sequencing (NGS) applications. Nucleic acid-based probes, such as peptide nucleic acid (PNA) oligomers, molecular beacons, DNA flares and locked nucleic acid (LNA) oligomers, are also provided for use in the methods and kits of the present disclosure.

Provided herein is a method for chemically capping polynucleotides having a 5′ monophosphate. In some embodiments the method may comprise: combining an activated nucleoside 5′ mono- or poly-phosphate with a population of polynucleotides that comprises polynucleotides having a 5′ monophosphate, to produce a reaction mix; and incubating the reaction mix to produce reaction products that comprise a polynucleotide and a 5′ nucleoside cap, linked by a 5′ to 5′ polyphosphate linkage. The chemical capping method described herein can be incorporated into a variety of cDNA synthesis methods.

The present disclosure relates to probes and methods for detecting nucleic acids, and for detecting and treating neovas-cularization.