The present invention relates to melting analysis based methods for detecting the presence of a variant sequence in a target nucleic acid sequence comprising nucleotides of interest, in particular to detect microsatellite instability. The methods employ probes as reporter oligonucleotides with fluorophore and quencher and wherein the nucleotide sequence comprises nucleotides with hydrophobic intercalating residues. Also disclosed are methods for determining efficacy of a drug and for predicting the presence of a clinical disorder in an individual, as well as reporter oligonucleotides and kits for performing the methods.

A method and system for obtaining and controlling genetic identification information are disclosed. The method includes providing personal information of a registrant to a secure website using an electronic communication device, taking a genetic material-containing sample from the registrant, providing the sample to a genetic material analysis facility, analyzing short tandem repeat (STR) regions of the genetic material at a plurality of loci to produce a genetic identity for the registrant, recording the personal information and the genetic identity in a blockchain ledger, and enabling the registrant to display on another electronic communication device a code corresponding to the genetic identity. The system includes a genetic material sampling kit, a short tandem repeat (STR) analysis kit, and electronic communication device(s) configured to enter the registrant's personal information, record the personal information and the genetic identity in the blockchain ledger, and display a code corresponding to the genetic identity.

A method and system for obtaining and controlling genetic identification information are disclosed. The method includes providing personal information of a registrant to a secure website using an electronic communication device, taking a genetic material-containing sample from the registrant, providing the sample to a genetic material analysis facility, analyzing short tandem repeat (STR) regions of the genetic material at a plurality of loci to produce a genetic identity for the registrant, recording the personal information and the genetic identity in a blockchain ledger, and enabling the registrant to display on another electronic communication device a code corresponding to the genetic identity. The system includes a genetic material sampling kit, a short tandem repeat (STR) analysis kit, and electronic communication device(s) configured to enter the registrant's personal information, record the personal information and the genetic identity in the blockchain ledger, and display a code corresponding to the genetic identity.

The present disclosure relates to nucleic acid probes and kits for determining the length of a region of tandem repeats in a subject's genome and methods of using the DNA probes for determining the length of a region of tandem repeats in a subject's genome. In some embodiments, the region of tandem repeats in telomeres.

The present disclosure relates to nucleic acid probes and kits for determining the length of a region of tandem repeats in a subject's genome and methods of using the DNA probes for determining the length of a region of tandem repeats in a subject's genome. In some embodiments, the region of tandem repeats in telomeres.

The present invention concerns new artificially synthesized single stranded nucleic acid molecules which may be used in many applications, and templates and methods for making the same. There are a multitude of uses for single stranded nucleic acid molecules, including but not limited to vectors for the delivery of sequences (for example a gene sequence, or a template for gene editing, gene knock-in or knock-down) or in bioengineering, for example as for constructing highly ordered materials from nanoparticle building blocks.

The present invention concerns new artificially synthesized single stranded nucleic acid molecules which may be used in many applications, and templates and methods for making the same. There are a multitude of uses for single stranded nucleic acid molecules, including but not limited to vectors for the delivery of sequences (for example a gene sequence, or a template for gene editing, gene knock-in or knock-down) or in bioengineering, for example as for constructing highly ordered materials from nanoparticle building blocks.

Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer.

Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer.

Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.