The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Provided herein are methods of encoding data on a polymer. Also provided are methods of reading data encoded on a polymer. Also provided are systems for encoding data on a polymer; systems for reading data encoded on a polymer; and data encoding/data reading platforms.

Provided herein are methods of encoding data on a polymer. Also provided are methods of reading data encoded on a polymer. Also provided are systems for encoding data on a polymer; systems for reading data encoded on a polymer; and data encoding/data reading platforms.

Disclosed are synthetic 71 base pair, partially-double stranded DNA oligonucleotides and methods for their use in the amplification and identification or integrated proviral DNA.

Disclosed are synthetic 71 base pair, partially-double stranded DNA oligonucleotides and methods for their use in the amplification and identification or integrated proviral DNA.

The present invention relates to a method for amplifying and detecting ribonucleic acid (RNA) fragments. In particular, the method of the present invention comprises conversion of RNA fragments to cDNA and DNA amplification. The present invention also provides a kit for performing the method as described herein.

The present invention relates to a method for amplifying and detecting ribonucleic acid (RNA) fragments. In particular, the method of the present invention comprises conversion of RNA fragments to cDNA and DNA amplification. The present invention also provides a kit for performing the method as described herein.

A non-specific-binding inhibitor enables, in detection of a target nucleic acid by hybridization, effective inhibition of cross-hybridization between the target nucleic acid and complementary strands of sequences similar thereto, which non-specific-binding inhibitor has a stable quality among production lots, and a hybridization method for nucleic acid uses the inhibitor. The non-specific-binding inhibitor for nucleic acid includes a nucleic acid which has a base length of 2 to 11 bases and in which the content of a guanine base(s) and/or methylated guanine base(s) in the entire base sequence is not less than 70%.

A non-specific-binding inhibitor enables, in detection of a target nucleic acid by hybridization, effective inhibition of cross-hybridization between the target nucleic acid and complementary strands of sequences similar thereto, which non-specific-binding inhibitor has a stable quality among production lots, and a hybridization method for nucleic acid uses the inhibitor. The non-specific-binding inhibitor for nucleic acid includes a nucleic acid which has a base length of 2 to 11 bases and in which the content of a guanine base(s) and/or methylated guanine base(s) in the entire base sequence is not less than 70%.