Determining the sequence of a nucleic acid typically entails performing multiple cycles of a reaction that generates a signal, depending on the identity of one or more nucleotides in the sequence. Sequencing typically is done on a plurality of copies of a template to fortify the signal and to increase accuracy. However, as the number of cycles increases, some of the copies go out of phase, increasing signal-to-noise ratio and compromising accuracy. Provided is a strategy using blocking groups and dinucleotide recognition to bring each of the copies back into phase. This improves accuracy and enables the user to increase the length of sequence reads.

The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.

The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.

The present disclosure provides improved nucleic acid sequencing-by-synthesis (SBS) methods, related kits and reagents, and systems for performing such methods using such kits and reagents.

Provided herein are probes in which an Ap site of a probe strand can selectively cross-link with a 2′-deoxyguanosine (dG) of a target strand. Also provided for are methods of using such probes to generate and detect crosslinked molecules and methods of detecting disease associated genetic mutations.

Provided herein are probes in which an Ap site of a probe strand can selectively cross-link with a 2′-deoxyguanosine (dG) of a target strand. Also provided for are methods of using such probes to generate and detect crosslinked molecules and methods of detecting disease associated genetic mutations.

This invention relates to methods for analyzing nucleic acids. The disclosure provides methods of a primer-dependent amplification and detection method that is capable of amplifying and detecting in a sample as few as ten copies of at least one rare intended target sequence in the presence of abundant closely related unintended target sequences. Also provided are reaction compositions and kits for performing the methods.

This invention relates to methods for analyzing nucleic acids. The disclosure provides methods of a primer-dependent amplification and detection method that is capable of amplifying and detecting in a sample as few as ten copies of at least one rare intended target sequence in the presence of abundant closely related unintended target sequences. Also provided are reaction compositions and kits for performing the methods.

The present invention relates to a method for determining a nucleotide sequence of a target nucleic acid. The method comprises: providing a pool of amplicons; sequencing each amplicon in the pool of amplicons to obtain sequence information of each amplicon; comparing a part of the sequence information of each amplicon with at least a part of the sequence of the target specific primer section, wherein the part of the sequence information of each amplicon is a sequence starting from position X+1; determining whether the part of the sequence information of each amplicon comprises at least the part of the sequence of the target specific primer section; and determining accurate sequence of the target region using sequence information which comprises at least the part of the sequence of the target-specific primer section.

The present invention relates to a method for determining a nucleotide sequence of a target nucleic acid. The method comprises: providing a pool of amplicons; sequencing each amplicon in the pool of amplicons to obtain sequence information of each amplicon; comparing a part of the sequence information of each amplicon with at least a part of the sequence of the target specific primer section, wherein the part of the sequence information of each amplicon is a sequence starting from position X+1; determining whether the part of the sequence information of each amplicon comprises at least the part of the sequence of the target specific primer section; and determining accurate sequence of the target region using sequence information which comprises at least the part of the sequence of the target-specific primer section.