This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.

A method for identifying a nucleic acid template that include (a) providing a plurality of primer-template hybrids, wherein a first hybrid of the plurality includes a first template hybridized to a first primer, and wherein a second hybrid of the plurality includes a second template hybridized to a second primer, the second primer having a ternary complex inhibitor moiety at the 3 end; (b) delivering polymerases and nucleotides to the plurality, whereby the first hybrid binds a polymerase and nucleotide to form a stabilized ternary complex and whereby the second hybrid does not bind a polymerase and nucleotide to form a stabilized ternary complex; and (c) detecting the stabilized ternary complex to identify the first template.

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.