The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.

The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.

The present application relates to the field the field of bioinformatics. Specifically, the present application relates to a method, system, electronic device and computer-readable medium for predicting the source of a sample to be tested based on multi-omics and multidimensional plasma features and artificial intelligence.

The present application relates to the field the field of bioinformatics. Specifically, the present application relates to a method, system, electronic device and computer-readable medium for predicting the source of a sample to be tested based on multi-omics and multidimensional plasma features and artificial intelligence.

Described herein is a method of sequencing a template that comprises a direct repeat, comprising: (a) in the same reaction, hybridizing a primer to a first site that is upstream of the first repeat sequence and hybridizing a primer to a second site that is upstream of the second repeat sequence, wherein the first and second sites are: (i) upstream of the first and second repeat sequences, respectively, and (ii) equidistant from the first and second repeat sequences; and (b) subjecting the hybridization product of (a) to a sequencing-by-synthesis sequencing reaction to produce a sequence read that comprises a combination of the first and second repeat sequences.

Described herein is a method of sequencing a template that comprises a direct repeat, comprising: (a) in the same reaction, hybridizing a primer to a first site that is upstream of the first repeat sequence and hybridizing a primer to a second site that is upstream of the second repeat sequence, wherein the first and second sites are: (i) upstream of the first and second repeat sequences, respectively, and (ii) equidistant from the first and second repeat sequences; and (b) subjecting the hybridization product of (a) to a sequencing-by-synthesis sequencing reaction to produce a sequence read that comprises a combination of the first and second repeat sequences.

Disclosed are methods of suppressing adaptor dimer formation comprising: providing a target polynucleotide with a 5′ and 3′ end; providing a double stranded DNA adaptor with a 5′ end and a 3′ end that have sequence complementary to each other, ligating the double stranded adaptor to the target polynucleotide to form a ligation product. Also provided is a method of preparing a library of nucleic acid sequences comprising: providing a double-stranded DNA adaptor with 5′ and 3′ ends having a sequence complementary to each other, contacting the adaptor with a target nucleic acid sequences having a 5′ and a 3′ end, and ligating the adaptor with complementary sequence to the 5′ and 3′ ends of the target nucleic acid sequence using a double stranded DNA ligase. The disclosure also provides kits for suppression of adaptor dimer formation in deep sequencing containing a double stranded DNA adaptor with 5′ and 3′ ends having a sequence complementary to each other, suitable enzymes, buffers, dNTPS, etc.

Disclosed are methods of suppressing adaptor dimer formation comprising: providing a target polynucleotide with a 5′ and 3′ end; providing a double stranded DNA adaptor with a 5′ end and a 3′ end that have sequence complementary to each other, ligating the double stranded adaptor to the target polynucleotide to form a ligation product. Also provided is a method of preparing a library of nucleic acid sequences comprising: providing a double-stranded DNA adaptor with 5′ and 3′ ends having a sequence complementary to each other, contacting the adaptor with a target nucleic acid sequences having a 5′ and a 3′ end, and ligating the adaptor with complementary sequence to the 5′ and 3′ ends of the target nucleic acid sequence using a double stranded DNA ligase. The disclosure also provides kits for suppression of adaptor dimer formation in deep sequencing containing a double stranded DNA adaptor with 5′ and 3′ ends having a sequence complementary to each other, suitable enzymes, buffers, dNTPS, etc.

The present description provides a cancer assay panel for targeted detection of cancer-specific methylation patterns. Further provided herein includes methods of designing, making, and using the cancer assay panel to detect cancer and particular types of cancer.

The present description provides a cancer assay panel for targeted detection of cancer-specific methylation patterns. Further provided herein includes methods of designing, making, and using the cancer assay panel to detect cancer and particular types of cancer.