Disclosed herein are methods and compositions for detecting Bordetella pertussis and Bordetella parapertussis by detecting the presence of the IS481 and IS1001 genomic insertion sequences, respectively.

The invention provides methods and materials that can be used to determine three dimensional structures of RNA hairpin loops and their complexes with inhibitors easily and quickly. The scaffold RNA, YdaO-type c-di-AMP riboswitch from Thermoanaerobacterpseudethanolicus, readily forms crystals with a large cavity over 60 in diameter. A hairpin of interest can be engineered into the P2 stem of this RNA so that the hairpin is accommodated in the cavity. The fusion RNA is then crystallized, and structures can be determined using X-ray or electron crystallography. Embodiments of the invention can be used to identify compounds that bind hairpin loops in order to, for example, effect therapeutic and other biological activities.

The invention provides methods and materials that can be used to determine three dimensional structures of RNA hairpin loops and their complexes with inhibitors easily and quickly. The scaffold RNA, YdaO-type c-di-AMP riboswitch from Thermoanaerobacterpseudethanolicus, readily forms crystals with a large cavity over 60 in diameter. A hairpin of interest can be engineered into the P2 stem of this RNA so that the hairpin is accommodated in the cavity. The fusion RNA is then crystallized, and structures can be determined using X-ray or electron crystallography. Embodiments of the invention can be used to identify compounds that bind hairpin loops in order to, for example, effect therapeutic and other biological activities.

The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.

The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.

Disclosed are means of stimulating systemic immunity and reduction of post-surgery tumor metastasis through the concurrent intralymphatic inhibition of NR2F6 and treatment with cannabidiol. In some embodiments NR2F6 is inhibited by high pressure transient delivery of short interfering RNA into tumor draining lymph nodes concurrent with systemic administration of cannabidiol. This combination may be performed together with means that induce immunogenic tumor cell death. Through the combination of immunogenic cell death and immune stimulation, the invention provides a means of enhancing the abscopal effect and in some embodiments to cause immunological mediated destruction primary and secondary neoplasia.

Provided herein is a method of moving a double-stranded polynucleotide with respect to a nanopore using a motor protein. The method allows a portion of the polynucleotide to be interrogated by the pore multiple times. Also provided are polynucleotide adapters and kits comprising such adapters. The methods find use in characterising polynucleotides, for example in sequencing.

Provided herein is a method of moving a double-stranded polynucleotide with respect to a nanopore using a motor protein. The method allows a portion of the polynucleotide to be interrogated by the pore multiple times. Also provided are polynucleotide adapters and kits comprising such adapters. The methods find use in characterising polynucleotides, for example in sequencing.

Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.

Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.