Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.

Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.

This invention relates to a bioassay method for detecting and/or quantitating a short single-stranded nucleic acid analyte employing a binary probe system, where at least one of the two discrete oligonucleotide probe parts of the binary probe has partially double-stranded (self-complementary) stem-loop structure at one terminus and single-stranded overhang sequence region at the other terminus, where the single-stranded terminal regions of both discrete parts of the binary probe hybridize to adjacent complementary regions in the sequence of the nucleic acid analyte molecule, and at least one discrete part of the binary probe comprising a stem-loop structure and single-stranded overhang sequence region hybridizes to terminal region in the sequence of the nucleic acid analyte molecule forming a nick structure. The binary probe system employed in the bioassay method is based on a luminescent reporter technology, either lanthanide chelate complementation or resonance energy transfer with lanthanide label as a donor. Thereby the method allows detection and/or quantitation of the short nucleic acid analyte molecule by time-resolved fluorometry.

This invention relates to a bioassay method for detecting and/or quantitating a short single-stranded nucleic acid analyte employing a binary probe system, where at least one of the two discrete oligonucleotide probe parts of the binary probe has partially double-stranded (self-complementary) stem-loop structure at one terminus and single-stranded overhang sequence region at the other terminus, where the single-stranded terminal regions of both discrete parts of the binary probe hybridize to adjacent complementary regions in the sequence of the nucleic acid analyte molecule, and at least one discrete part of the binary probe comprising a stem-loop structure and single-stranded overhang sequence region hybridizes to terminal region in the sequence of the nucleic acid analyte molecule forming a nick structure. The binary probe system employed in the bioassay method is based on a luminescent reporter technology, either lanthanide chelate complementation or resonance energy transfer with lanthanide label as a donor. Thereby the method allows detection and/or quantitation of the short nucleic acid analyte molecule by time-resolved fluorometry.

Disclosed herein is a transmembrane nanosensor device comprising a lipid conjugated DNA tweezer, and methods of using the same.

Disclosed herein is a transmembrane nanosensor device comprising a lipid conjugated DNA tweezer, and methods of using the same.

The present invention relates to a utilizing method of a nucleic acid compound containing a selectively cleavable site. Also, the present invention relates to a DNA-encoded library containing the selectively cleavable site, a composition for synthesis therefor and a method of use thereof.

The present invention relates to a utilizing method of a nucleic acid compound containing a selectively cleavable site. Also, the present invention relates to a DNA-encoded library containing the selectively cleavable site, a composition for synthesis therefor and a method of use thereof.

Systems and methods for determining the presence or absence of an analyte including a nucleic acid (e.g., DNA and RNA), a small molecule (e.g., proteins and amino acid chains), and one or more electrolytes (e.g., Na.sup.+ and K.sup.+). The system or method may detect multiple analytes (e.g., a first DNA and a second DNA) and/or multiple types of analytes (e.g., an RNA and an antibody protein). The signal readout provided by the system or method may be readily understood and may be correlated with a health condition (e.g., hydration or exposure to an infectious agent). The system may be wearable and may analyze one or more biofluids.

Systems and methods for determining the presence or absence of an analyte including a nucleic acid (e.g., DNA and RNA), a small molecule (e.g., proteins and amino acid chains), and one or more electrolytes (e.g., Na.sup.+ and K.sup.+). The system or method may detect multiple analytes (e.g., a first DNA and a second DNA) and/or multiple types of analytes (e.g., an RNA and an antibody protein). The signal readout provided by the system or method may be readily understood and may be correlated with a health condition (e.g., hydration or exposure to an infectious agent). The system may be wearable and may analyze one or more biofluids.