A gene analyzer has a structure in which a metal block, a heater installed on an outer surface of the metal block, and a heat insulator applied onto an outer surface of the heater are integrally formed, thereby miniaturizing the gene analyzer. Further, parts for light measurement, such as a light source element, a light-receiving element, a light source filter, a fluorescent filter, a light source lens, a fluorescent lens, and the like, are installed in a heat insulator light source path and a heat insulator fluorescent path formed in the heat insulator, and the paths formed in the heat insulator are aligned with a block light source path and a block fluorescent path of the metal block.

The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.

The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.

An automated multiplex detector system includes: (a) a nucleic acid amplification compartment for amplifying nucleic acid of one or more targets in a sample, and (b) an analysis compartment in fluid communication with the amplification compartment, the analysis compartment housing a nanoparticle-based multiplex detector capable of using the amplified nucleic acid of the amplification compartment and producing a signal that correlates with the presence of the one or more targets in the sample.

An automated multiplex detector system includes: (a) a nucleic acid amplification compartment for amplifying nucleic acid of one or more targets in a sample, and (b) an analysis compartment in fluid communication with the amplification compartment, the analysis compartment housing a nanoparticle-based multiplex detector capable of using the amplified nucleic acid of the amplification compartment and producing a signal that correlates with the presence of the one or more targets in the sample.

Provided is a device including a well provided in a number of at least one, wherein a nucleic acid having at least one of a full-length nucleotide sequence and a partial nucleotide sequence of rRNA or rDNA is contained in a defined copy number in at least one well of the well, and wherein the defined copy number of the nucleic acid having at least one of a full-length nucleotide sequence and a partial nucleotide sequence of rRNA or rDNA is 1,000 or less.

Provided is a device including a well provided in a number of at least one, wherein a nucleic acid having at least one of a full-length nucleotide sequence and a partial nucleotide sequence of rRNA or rDNA is contained in a defined copy number in at least one well of the well, and wherein the defined copy number of the nucleic acid having at least one of a full-length nucleotide sequence and a partial nucleotide sequence of rRNA or rDNA is 1,000 or less.

High throughput methods utilizing spatially segregated detection systems provide a robust CRISPR-based diagnostic enabling highly sensitive detection of both DNA and RNA target molecules, with applications in multiple scenarios in human health including, for example, viral detection. Kits comprising the systems allow for point-of care applications with high-throughput processing of samples.

High throughput methods utilizing spatially segregated detection systems provide a robust CRISPR-based diagnostic enabling highly sensitive detection of both DNA and RNA target molecules, with applications in multiple scenarios in human health including, for example, viral detection. Kits comprising the systems allow for point-of care applications with high-throughput processing of samples.

The present invention relates to a method for three-dimensional nucleic acid imaging diagnosis of a tissue by using isothermal nucleic acid amplification. The method for three-dimensional nucleic acid imaging diagnosis of tissue according to the present invention can allow a specific molecular biomarker to be clearly seen in tissue through clearing of the tissue, enhance diagnostic accuracy by three-dimensionally reconstituting the tissue since all of the inside of the tissue is visualized, and facilitate three-dimensional imaging of a molecular biomarker such as DNA or RNA containing genetic information in the human body through isothermal nucleic acid amplification in tissue, and thus this method can be effectively used in diagnosis of various diseases including cancer.