Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

Provided herein are methods for spatial analysis using targeted RNA depletion.

Provided herein are methods for spatial analysis using targeted RNA depletion.

A device for rapid non-destructive genetic material collection can include a multi-reservoir array (202) and a movement mechanism. The multi-reservoir array (202) can include multiple reservoirs (204). A plurality of the multiple reservoirs (204) can include an abrasive surface (210) capable of retaining a source of genetic material in a liquid carrier. The abrasive surface (210) has a roughness. The movement mechanism can be operable to move the multi-reservoir array (202) in an oscillating motion sufficient to create relative movement between the abrasive surface (210) and the source of the genetic material in order to remove a portion of genetic material from the source of the genetic material without destroying the source of the genetic material or the portion of the genetic material that is removed.

A device for rapid non-destructive genetic material collection can include a multi-reservoir array (202) and a movement mechanism. The multi-reservoir array (202) can include multiple reservoirs (204). A plurality of the multiple reservoirs (204) can include an abrasive surface (210) capable of retaining a source of genetic material in a liquid carrier. The abrasive surface (210) has a roughness. The movement mechanism can be operable to move the multi-reservoir array (202) in an oscillating motion sufficient to create relative movement between the abrasive surface (210) and the source of the genetic material in order to remove a portion of genetic material from the source of the genetic material without destroying the source of the genetic material or the portion of the genetic material that is removed.

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

The present invention disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the invention is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The invention includes one or more target-specific nucleic acid probes per genetic target and a bridge oligo or bridge oligo complex.

The present invention disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the invention is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The invention includes one or more target-specific nucleic acid probes per genetic target and a bridge oligo or bridge oligo complex.