Bistable devices are constructed using a polynucleotide platform for sensing molecular events such as binding or conformational changes of target molecules. Uses include measurement of target concentration, measuring the effect of environmental condition (such as heat, light, or pH) on the target, or screening a library for molecules that bind the target or modulate its biological function. Devices comprise three regions: a top lid, bottom lid, and flexible linker or hinge between them. A device has an open configuration in which the top and bottom lid are separated, and a closed configuration they are bound close together. Binding domains or variations of the target molecule are fixed to a device so that when the molecular event occurs, the device switches from open to closed, or vice versa, which generates a signal. Optimal device design is determined by the signal modality (optical or electronic) used to measure closure of surface-immobilized devices.

Bistable devices are constructed using a polynucleotide platform for sensing molecular events such as binding or conformational changes of target molecules. Uses include measurement of target concentration, measuring the effect of environmental condition (such as heat, light, or pH) on the target, or screening a library for molecules that bind the target or modulate its biological function. Devices comprise three regions: a top lid, bottom lid, and flexible linker or hinge between them. A device has an open configuration in which the top and bottom lid are separated, and a closed configuration they are bound close together. Binding domains or variations of the target molecule are fixed to a device so that when the molecular event occurs, the device switches from open to closed, or vice versa, which generates a signal. Optimal device design is determined by the signal modality (optical or electronic) used to measure closure of surface-immobilized devices.

The present invention relates to methods for detecting and quantifying intact protein-polynucleotide conjugate molecules in various sample matrices. In particular, the methods utilize triplex forming oligonucleotides in combination with protein-specific binding partners to respectively detect the polynucleotide and protein components of the conjugate molecules.

The present invention relates to methods for detecting and quantifying intact protein-polynucleotide conjugate molecules in various sample matrices. In particular, the methods utilize triplex forming oligonucleotides in combination with protein-specific binding partners to respectively detect the polynucleotide and protein components of the conjugate molecules.

Disclosed herein are compositions, kits, methods, and systems for detecting a target molecule in a sample using a detection molecule.

Disclosed herein are compositions, kits, methods, and systems for detecting a target molecule in a sample using a detection molecule.

A method for detecting a target nucleic acid fragment includes the following steps: preparing a nucleic acid probe and an intercalating dye configured to bind to double-stranded nucleic acids, wherein the nucleic acid probe includes a nucleic acid chain, a fluorescent reporter, and a fluorescent quencher, the fluorescent reporter is conjugated to a first end of the nucleic acid chain, and the fluorescent quencher is conjugated to a second end of the nucleic acid chain opposing to the first end; binding the nucleic acid probe to the target nucleic acid fragment to form a partial double stranded structure; intercalating the intercalating dye in the partial double stranded structure, so that, after the intercalating dye is excited by an irradiation, the fluorescent reporter is excited through the intercalating dye to emit a fluorescence signal; and detecting the target nucleic acid fragment according to the fluorescence signal.

A method for detecting a target nucleic acid fragment includes the following steps: preparing a nucleic acid probe and an intercalating dye configured to bind to double-stranded nucleic acids, wherein the nucleic acid probe includes a nucleic acid chain, a fluorescent reporter, and a fluorescent quencher, the fluorescent reporter is conjugated to a first end of the nucleic acid chain, and the fluorescent quencher is conjugated to a second end of the nucleic acid chain opposing to the first end; binding the nucleic acid probe to the target nucleic acid fragment to form a partial double stranded structure; intercalating the intercalating dye in the partial double stranded structure, so that, after the intercalating dye is excited by an irradiation, the fluorescent reporter is excited through the intercalating dye to emit a fluorescence signal; and detecting the target nucleic acid fragment according to the fluorescence signal.