The invention relates to a system (10) for the amplification of a nucleic acid (22), comprising at least one local heating element (12), which is functionalized with at least one connection nucleic acid (14), and at least one primer nucleic acid (16), which is adapted to bind to the at least one connection nucleic acid (14) and to bind to the nucleic acid (22), and/or at least one primer complementary nucleic acid (30), which is adapted to bind to the at least one connection nucleic acid (14) and to elongate the connection nucleic acid (14) by a primer nucleotide sequence by means of an enzymatic reaction. Furthermore, the invention relates to a primer nucleic acid (16), a primer complementary nucleic acid (30), a local heating element (12) and a method for the amplification of a nucleic acid (22).

The invention relates to a system (10) for the amplification of a nucleic acid (22), comprising at least one local heating element (12), which is functionalized with at least one connection nucleic acid (14), and at least one primer nucleic acid (16), which is adapted to bind to the at least one connection nucleic acid (14) and to bind to the nucleic acid (22), and/or at least one primer complementary nucleic acid (30), which is adapted to bind to the at least one connection nucleic acid (14) and to elongate the connection nucleic acid (14) by a primer nucleotide sequence by means of an enzymatic reaction. Furthermore, the invention relates to a primer nucleic acid (16), a primer complementary nucleic acid (30), a local heating element (12) and a method for the amplification of a nucleic acid (22).

Provided includes methods and systems useful in array-based analysis of mixed nucleic acid populations, including for genotyping and copy number analysis of the various subpopulations of the mixed nucleic acid population. Also provided includes methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken from an organism.

Provided includes methods and systems useful in array-based analysis of mixed nucleic acid populations, including for genotyping and copy number analysis of the various subpopulations of the mixed nucleic acid population. Also provided includes methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken from an organism.

Provided herein is technology relating to detecting and identifying nucleic acids and particularly, but not exclusively, to compositions, methods, kits, and systems for detecting, identifying, and quantifying target nucleic acids with high confidence at single-molecule resolution.

Provided herein is technology relating to detecting and identifying nucleic acids and particularly, but not exclusively, to compositions, methods, kits, and systems for detecting, identifying, and quantifying target nucleic acids with high confidence at single-molecule resolution.

The present invention provides a method for detecting a plurality of analytes in a sample, comprising performing a multiplex proximity-based detection assay. The assay utilises pairs of proximity probes with shared hybridisation sites (i.e. hybridisation sites which are shared between different proximity probe pairs). Also provided is a product comprising a plurality of proximity probe pairs with shared hybridisation sites, which may be used in the method disclosed herein.

The present invention provides a method for detecting a plurality of analytes in a sample, comprising performing a multiplex proximity-based detection assay. The assay utilises pairs of proximity probes with shared hybridisation sites (i.e. hybridisation sites which are shared between different proximity probe pairs). Also provided is a product comprising a plurality of proximity probe pairs with shared hybridisation sites, which may be used in the method disclosed herein.

Devices and kits for detecting molecular targets involved in the onset of parturition are presented, as are methods of using the same. The devices and kits include one or more associators specific for molecular targets, such as microRNAs and proteins and peptide fragments thereof. The associators, such as aptamers or antibodies, bind their targets, when such targets are present in a fluid sample from a pregnant woman, to produce a detectable signal. The signal may be produced in a lateral flow device.

Kits and methods of using the kits for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding are disclosed. The sample analysis kits employ nucleic acid encoding and/or nucleic acid recording of a molecular interaction and/or reaction, such as recognition events (e.g., between an antigen and an antibody, between a modified terminal amino acid residue, or between a small molecule or peptide therapeutic and a target, etc.). Additional barcoding reagents, such as those for cycle-specific barcoding (e.g., “clocking”), compartment barcoding, combinatorial barcoding, spatial barcoding, or any combination thereof, may be included in the kits. The sample may comprise macromolecules, including peptides, polypeptides, and proteins, and the recording may generate molecular interaction and/or reaction information, and/or polypeptide sequence information. The kits may be used in high-throughput, multiplexed, and/or automated analysis, and are suitable for analysis of a proteome or subset thereof.