A method of detecting a target molecule, the method comprising: forming a complex of a target molecule, a capture oligonucleotide, an oligonucleotide primer, and a single-stranded circular DNA; performing a nucleic acid amplification reaction by rolling circle amplification based on the formation of the complex; and detecting amplified nucleic acid; wherein the single-stranded circular DNA contains a first region, and a second region linked to the 3′-side of the first region, and preferably further contains a sequence complementary to a detection reagent-binding sequence; the primer contains a first aptamer sequence which binds to the target molecule, and a sequence which is linked to the 3′-side of the first aptamer sequence and is complementary to the first region of the single-stranded circular DNA; and the capture oligonucleotide contains a sequence complementary to the second region of the single-stranded circular DNA, and a second aptamer sequence which is linked to the 3′-side of the sequence complementary to the second region and binds to the target molecule.

The present invention relates to methods for detecting and quantifying intact protein-polynucleotide conjugate molecules in various sample matrices. In particular, the methods utilize triplex forming oligonucleotides in combination with protein-specific binding partners to respectively detect the polynucleotide and protein components of the conjugate molecules.

The present invention relates to methods for detecting and quantifying intact protein-polynucleotide conjugate molecules in various sample matrices. In particular, the methods utilize triplex forming oligonucleotides in combination with protein-specific binding partners to respectively detect the polynucleotide and protein components of the conjugate molecules.

The invention inter alia pertains to methods and kits for analysis of one or more cell released biomolecules. Furthermore, the invention relates to a plurality of sequenceable products comprising different sequence elements. The described technology is useful for a variety of applications, in particular biomolecule analysis applications, e.g. for obtaining biomolecule release profiles of single cells in a multiplexed manner, wherein the cells are provided in a matrix

The invention inter alia pertains to methods and kits for analysis of one or more cell released biomolecules. Furthermore, the invention relates to a plurality of sequenceable products comprising different sequence elements. The described technology is useful for a variety of applications, in particular biomolecule analysis applications, e.g. for obtaining biomolecule release profiles of single cells in a multiplexed manner, wherein the cells are provided in a matrix

An assay for detecting gene fragments, including: amplifying gene fragments comprising single-nucleotide polymorphisms (SNPs) to form an initial amplification product; isolating single strand oligonucleotides of interest from the initial amplification product; forming a solution comprising the oligonucleotides of interest and reporter molecules, each reporter molecule having a first oligonucleotide domain configured to hybridize with a complementary oligonucleotide of interest, and a second oligonucleotide domain configured to hybridize with a complementary capture probe; hybridizing, in the solution, the oligonucleotides of interest with the reporter molecules that have complementary first domains; applying the solution to the surface of a microarray including an array of capture probes fixed to a microarray slide; capturing the oligonucleotides of interest on the microarray by hybridizing the second oligonucleotide domains of the reporter molecules with complementary capture probes of the microarray; and detecting the hybridized oligonucleotides of interest captured on the microarray.

An assay for detecting gene fragments, including: amplifying gene fragments comprising single-nucleotide polymorphisms (SNPs) to form an initial amplification product; isolating single strand oligonucleotides of interest from the initial amplification product; forming a solution comprising the oligonucleotides of interest and reporter molecules, each reporter molecule having a first oligonucleotide domain configured to hybridize with a complementary oligonucleotide of interest, and a second oligonucleotide domain configured to hybridize with a complementary capture probe; hybridizing, in the solution, the oligonucleotides of interest with the reporter molecules that have complementary first domains; applying the solution to the surface of a microarray including an array of capture probes fixed to a microarray slide; capturing the oligonucleotides of interest on the microarray by hybridizing the second oligonucleotide domains of the reporter molecules with complementary capture probes of the microarray; and detecting the hybridized oligonucleotides of interest captured on the microarray.

Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

Among other things, the present disclosure is related to devices and methods of performing biological and chemical assays, such as but not limited to immunoassays and nucleic assay acid, particularly a homogeneous assay that does not use a wash step and that is fast (e.g., 60 seconds from dropping a sample to displaying results). The present disclosure is related to both competitive and non-competitive homogeneous assays.