The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

The present invention relates to a method of obtaining an enriched personalized population of a target polynucleotide using a synthetic single guide RNA (sgRNA) for an sgRNA-guided nucleic acid-binding protein, as well as to a method of obtaining a pool of personalized target-irrelevant synthetic single guide RNAs (sgRNAs) for a sgRNA-guided nucleic acid-binding protein. Also provided is a kit comprising a pool of sgRNAs obtainable by the methods of the invention, the use of a pool of sgRNAs obtainable by the methods of the invention and a method of monitoring a disease state.

The present invention relates to a method of obtaining an enriched personalized population of a target polynucleotide using a synthetic single guide RNA (sgRNA) for an sgRNA-guided nucleic acid-binding protein, as well as to a method of obtaining a pool of personalized target-irrelevant synthetic single guide RNAs (sgRNAs) for a sgRNA-guided nucleic acid-binding protein. Also provided is a kit comprising a pool of sgRNAs obtainable by the methods of the invention, the use of a pool of sgRNAs obtainable by the methods of the invention and a method of monitoring a disease state.

The invention is a method of assessing a mammalian immune repertoire by primer extension target enrichment (PETE). Methods and compositions for assessing an immune repertoire and the status of additional genetic markers are disclosed.

The invention is a method of assessing a mammalian immune repertoire by primer extension target enrichment (PETE). Methods and compositions for assessing an immune repertoire and the status of additional genetic markers are disclosed.

Embodiments of the disclosure are drawn to apparatuses, systems, and methods for enrichment and separation of nucleic acids by size. A sample may include a mixture of nucleic acids of various sizes, and the nucleic acids of interest may be below a particular size threshold. An example enrichment method may include mixing the sample with a first substrate (e.g., magnetic beads). The method may include separating nucleic acids above a first size threshold form a remainder of the sample using the first substrate. The method may include mixing the nucleic acids in the remainder of the sample (e.g., nucleic acids below’ the size threshold) with a second substrate and recovering the nucleic acids below the first size threshold from the second substrate.

Embodiments of the disclosure are drawn to apparatuses, systems, and methods for enrichment and separation of nucleic acids by size. A sample may include a mixture of nucleic acids of various sizes, and the nucleic acids of interest may be below a particular size threshold. An example enrichment method may include mixing the sample with a first substrate (e.g., magnetic beads). The method may include separating nucleic acids above a first size threshold form a remainder of the sample using the first substrate. The method may include mixing the nucleic acids in the remainder of the sample (e.g., nucleic acids below’ the size threshold) with a second substrate and recovering the nucleic acids below the first size threshold from the second substrate.

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.