Disclosed is a method of quantitatively analyzing a target protein population in a sample to be analyzed, the method including (a) treating a sample to be analyzed with an aptamer library specific to a target protein population present in the sample so as to form complexes between target proteins and aptamers binding specifically thereto, thereby forming a target protein-aptamer complex population, (b) isolating the complex population from unbound aptamers, and (c) analyzing the sequence of each aptamer of the complex population through a next-generation sequencing process so as to quantify each aptamer of the complex population, thereby quantifying each target protein in the complex population. The method of the present invention can be very useful in collectively quantifying proteins in an analytical sample.

This disclosure describes methods and compositions for protein and peptide sequencing.

This disclosure describes methods and compositions for protein and peptide sequencing.

Methods and compositions are provided to identify oligonucleotides that bind targets of interest. The targets include tissues, cells, circulating biomarkers such as microvesicles, including those derived from various diseases. The oligonucleotides can be used in diagnostic and therapeutic applications.

Methods and compositions are provided to identify oligonucleotides that bind targets of interest. The targets include tissues, cells, circulating biomarkers such as microvesicles, including those derived from various diseases. The oligonucleotides can be used in diagnostic and therapeutic applications.

The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art.

The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art.

Methods for selecting single or multiple aptamer pairs against target molecules in free solution have been developed. These methods utilize novel cooperative evolution approaches to select aptamer pairs against one or more targets, in which the pairing of one or more aptamers upon target binding triggers aptamer amplifiability. In this manner, the enrichment of aptamer ligands through one or multiple rounds of the selection process is based predominantly upon target-driven close proximity of aptamers in free solution. Target binding and enrichment are coupled using either positive or negative selection methods. These techniques should be generally applicable to many different types of target molecules, providing alternatives to antibodies, drugs, or other binding molecules for analytical, preparative, and therapeutic purposes.

Methods for selecting single or multiple aptamer pairs against target molecules in free solution have been developed. These methods utilize novel cooperative evolution approaches to select aptamer pairs against one or more targets, in which the pairing of one or more aptamers upon target binding triggers aptamer amplifiability. In this manner, the enrichment of aptamer ligands through one or multiple rounds of the selection process is based predominantly upon target-driven close proximity of aptamers in free solution. Target binding and enrichment are coupled using either positive or negative selection methods. These techniques should be generally applicable to many different types of target molecules, providing alternatives to antibodies, drugs, or other binding molecules for analytical, preparative, and therapeutic purposes.

According to one embodiment, a sensor includes an ionic liquid, a probe molecule, and a sensor element. The probe molecule selectively associates with a designated substance in the ionic liquid. The sensor element detects an association of the probe molecule with the designated substance.