This disclosure provides, among other things, a reagent system for nucleic acid analysis. In some embodiments, the system may comprise a plurality of oligonucleotide sets each set comprising at least (a) a competitor oligonucleotide that hybridizes to a target sequence and varies in concentration from mixture to mixture and (b) a detector oligonucleotide that also hybridizes to the target sequence and contains a barcode that indicates the concentration of the competitor oligonucleotide in the oligonucleotide set. The reagent system may be used to analyze a nucleic acid sample.

This disclosure provides, among other things, a reagent system for nucleic acid analysis. In some embodiments, the system may comprise a plurality of oligonucleotide sets each set comprising at least (a) a competitor oligonucleotide that hybridizes to a target sequence and varies in concentration from mixture to mixture and (b) a detector oligonucleotide that also hybridizes to the target sequence and contains a barcode that indicates the concentration of the competitor oligonucleotide in the oligonucleotide set. The reagent system may be used to analyze a nucleic acid sample.

The present invention provides a device and method for testing a material for the presence of DNA. The system includes a centrifuge, a microchip performing cell lysis and an enclosure that contains an isothermal ballast material and chromogenic agent that melts at a specific temperature and displays a color change, respectively.

The present invention provides a device and method for testing a material for the presence of DNA. The system includes a centrifuge, a microchip performing cell lysis and an enclosure that contains an isothermal ballast material and chromogenic agent that melts at a specific temperature and displays a color change, respectively.

The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

Described herein is an integrated automated nucleic acid sample preparation, detection and analysis system, along with methods of operating such a system. Also described are methods of methods of analyzing nucleic acid molecules in a sample and methods of determining a melting curve of a nucleic acid sample. The automated system includes a robotic pipettor comprising one or more pipettes movable in a horizontal plane and configured to dispense or withdraw one or more liquids, a robotic arm configured to transport a plurality of connected sample processing tubes, a nucleic acid isolation system comprising a first sample processing tube holder configured to hold the plurality of connected sample processing tubes, and magnet, wherein magnetically-responsive particles contained within each sample processing tube are retained within the sample processing tube upon withdrawing of liquid by the robotic pipettor when the magnet is in an active configuration; and a fluorometer comprising a light source and an optical detector disposed below a second sample processing tube holder configured to hold the plurality of connected sample processing tubes, the second sample processing tube holder having a clear or open bottom, wherein the fluorometer is configured to detect fluorescence emitted from a sample in one or more of the sample processing tubes.

Described herein is an integrated automated nucleic acid sample preparation, detection and analysis system, along with methods of operating such a system. Also described are methods of methods of analyzing nucleic acid molecules in a sample and methods of determining a melting curve of a nucleic acid sample. The automated system includes a robotic pipettor comprising one or more pipettes movable in a horizontal plane and configured to dispense or withdraw one or more liquids, a robotic arm configured to transport a plurality of connected sample processing tubes, a nucleic acid isolation system comprising a first sample processing tube holder configured to hold the plurality of connected sample processing tubes, and magnet, wherein magnetically-responsive particles contained within each sample processing tube are retained within the sample processing tube upon withdrawing of liquid by the robotic pipettor when the magnet is in an active configuration; and a fluorometer comprising a light source and an optical detector disposed below a second sample processing tube holder configured to hold the plurality of connected sample processing tubes, the second sample processing tube holder having a clear or open bottom, wherein the fluorometer is configured to detect fluorescence emitted from a sample in one or more of the sample processing tubes.