The present disclosure provides methods and systems for sequencing nucleic acid molecules in a manner that enables higher sequencing accuracy. Methods and systems provided herein may enable sequences that may have low-accuracy reads, such as homopolymer sequences or other repeating sequences, to be determined at a higher accuracy and efficiency.

A method and system to decode information stored on a polymer sequence, such as a DNA strand, is described herein. The method and system use molecular probes to label sections of the polymer sequence. Each molecular probe includes a fluorophore and a quencher. The fluorophore produces light with a color and wavelength corresponding to the information stored on the section of the polymer sequence the molecular probe labels. The quencher inhibits the production of light by an adjacent fluorophore. When adjacent sections of the polymer sequence are labeled with molecular probes, the fluorophore of the leading molecular probe produces light while the trailing molecular probe's light is quenched. The method and system then sequentially unbind the molecular probes from the sections of the polymer sequence within a waveguide, producing a sequence of observable fluorescence signals. The sequence can be used to determine the information stored on a polymer sequence.

A method and system to decode information stored on a polymer sequence, such as a DNA strand, is described herein. The method and system use molecular probes to label sections of the polymer sequence. Each molecular probe includes a fluorophore and a quencher. The fluorophore produces light with a color and wavelength corresponding to the information stored on the section of the polymer sequence the molecular probe labels. The quencher inhibits the production of light by an adjacent fluorophore. When adjacent sections of the polymer sequence are labeled with molecular probes, the fluorophore of the leading molecular probe produces light while the trailing molecular probe's light is quenched. The method and system then sequentially unbind the molecular probes from the sections of the polymer sequence within a waveguide, producing a sequence of observable fluorescence signals. The sequence can be used to determine the information stored on a polymer sequence.

The invention is directed to a method for determining a monomer sequence of a polymer that is translocated through a nanopore. Monomers of the polymer are labeled with fluorescent labels such that in free solution fluorescent labels of adjacent monomers substantially quench each other and wherein the nanopore constrains fluorescent labels within its bore into a constrained state wherein no detectable fluorescent signal can be generated. By exciting the fluorescent label of each monomer as it exits the nanopore and transitions from a constrained state to a quenched state with an adjacent fluorescent label, a fluorescent signal can be generated by the exiting fluorescent label that allows its monomer to be identified, thereby permitting a monomer sequence to be determined from a sequence of fluorescent signals as the polymer translocates through the nanopore.

The invention is directed to a method for determining a monomer sequence of a polymer that is translocated through a nanopore. Monomers of the polymer are labeled with fluorescent labels such that in free solution fluorescent labels of adjacent monomers substantially quench each other and wherein the nanopore constrains fluorescent labels within its bore into a constrained state wherein no detectable fluorescent signal can be generated. By exciting the fluorescent label of each monomer as it exits the nanopore and transitions from a constrained state to a quenched state with an adjacent fluorescent label, a fluorescent signal can be generated by the exiting fluorescent label that allows its monomer to be identified, thereby permitting a monomer sequence to be determined from a sequence of fluorescent signals as the polymer translocates through the nanopore.

There are provided methods and systems for detecting and/or quantifying an analyte. In particular, there are provided methods and systems for simultaneous detection and/or quantitation of two or more analytes in a sample. Colocalization-by-linkage assays on microparticles (CLAMP) can be engineered and used to effectively multiplex the detection of analytes within a sample. Features and methods of CLAMP systems can provide robust and scalable analysis of analytes in a sample.

There are provided methods and systems for detecting and/or quantifying an analyte. In particular, there are provided methods and systems for simultaneous detection and/or quantitation of two or more analytes in a sample. Colocalization-by-linkage assays on microparticles (CLAMP) can be engineered and used to effectively multiplex the detection of analytes within a sample. Features and methods of CLAMP systems can provide robust and scalable analysis of analytes in a sample.

A process for analysing chromosome regions and interactions relating to prognosis of Autism Spectrum Disorder.

A process for analysing chromosome regions and interactions relating to prognosis of Autism Spectrum Disorder.

Described herein are systems and methods that may be used to differentially detect viral serotype specific nucleic acid. For example, these systems may comprise multiple DNA-nanostructures, capture oligonucleotides and protector oligonucleotides, wherein each DNA-nanostructure and its associated capture oligonucleotide and protector oligonucleotide are specific for a unique viral type or serotype.