The present invention encompasses a diagnostic test and method to authenticate the veracity of a vaccine. The diagnostic test and method are especially useful in a specific and sensitive immunochromatographic assay, performable within about 15 minutes for the authentication, validation, and veracity of a vaccine, such as a COVID-19 vaccine, in a vial prior to administration to a human.

The present invention relates to melting analysis based methods for detecting the presence of a variant sequence in a target nucleic acid sequence comprising nucleotides of interest, in particular to detect microsatellite instability. The methods employ probes as reporter oligonucleotides with fluorophore and quencher and wherein the nucleotide sequence comprises nucleotides with hydrophobic intercalating residues. Also disclosed are methods for determining efficacy of a drug and for predicting the presence of a clinical disorder in an individual, as well as reporter oligonucleotides and kits for performing the methods.

The present invention relates to melting analysis based methods for detecting the presence of a variant sequence in a target nucleic acid sequence comprising nucleotides of interest, in particular to detect microsatellite instability. The methods employ probes as reporter oligonucleotides with fluorophore and quencher and wherein the nucleotide sequence comprises nucleotides with hydrophobic intercalating residues. Also disclosed are methods for determining efficacy of a drug and for predicting the presence of a clinical disorder in an individual, as well as reporter oligonucleotides and kits for performing the methods.

A structural molecular building block is provided and includes first structural molecules arranged in a three-dimensional structure and second structural molecules. Each of the second structural molecules is attached at a first region thereof to one of the first structural molecules to form the three-dimensional structure into a tessellating molecular building block and has a second region thereof for connection to a corresponding structural molecule of an additional tessellating molecular building block. The second structural molecules facilitate tessellation of the tessellating molecular building block with additional tessellating molecular building blocks to encourage growth of a macroscopic crystal.

A structural molecular building block is provided and includes first structural molecules arranged in a three-dimensional structure and second structural molecules. Each of the second structural molecules is attached at a first region thereof to one of the first structural molecules to form the three-dimensional structure into a tessellating molecular building block and has a second region thereof for connection to a corresponding structural molecule of an additional tessellating molecular building block. The second structural molecules facilitate tessellation of the tessellating molecular building block with additional tessellating molecular building blocks to encourage growth of a macroscopic crystal.

Diaryl-azo derivatives are efficient fluorescence quenchers as well as nucleic acid duplex-stabilizing agents and are useful in oligonucleotide conjugates and probes. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.

Diaryl-azo derivatives are efficient fluorescence quenchers as well as nucleic acid duplex-stabilizing agents and are useful in oligonucleotide conjugates and probes. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.

The present disclosure concerns a method for detecting and characterizing a virus comprising the steps of providing a sample to analyze that is likely to contain a virus, extracting and preparing nucleic acids from the sample, sequence-specifically labeling the nucleic acid, e.g. by introducing fluorophores by contacting the nucleic acid with a methyltransferase or by introducing fluorescently labelled nucleotides after treatment with nickase, performing a genomic mapping analysis of the extracted nucleic acids and performing a computational analysis to detect the presence of and characterise at least one virus. The present disclosure also concerns a kit for carrying out the above method.

The present disclosure concerns a method for detecting and characterizing a virus comprising the steps of providing a sample to analyze that is likely to contain a virus, extracting and preparing nucleic acids from the sample, sequence-specifically labeling the nucleic acid, e.g. by introducing fluorophores by contacting the nucleic acid with a methyltransferase or by introducing fluorescently labelled nucleotides after treatment with nickase, performing a genomic mapping analysis of the extracted nucleic acids and performing a computational analysis to detect the presence of and characterise at least one virus. The present disclosure also concerns a kit for carrying out the above method.

Light harvesting luminescent multichromophores that are configured upon excitation to transfer energy to, and amplify the emission from, an acceptor signaling chromophore in energy-receiving proximity therewith are provided. Also provided are compositions for labelling a target. The labelling composition may include a donor light harvesting multichromophore and an acceptor signaling chromophore in energy-receiving proximity to the donor light harvesting multichromophore. Also provided is an aqueous composition for labelling a target, including: a donor light harvesting multichromophore; an acceptor signaling chromophore in energy-receiving proximity therewith; and a sensor biomolecule. Methods for using the subject compositions are also provided.