This disclosure provides for apparatuses, systems and methods for in vitro sample detection. For example in one embodiment, this disclosure provides an automated Pe-toner microfluidic device (and related method) on a centrifugal platform for DNA sample lysis and DNA extraction. A second embodiment provides a system and method for qualitative detection, quantification, and real-time monitoring of nucleic acid amplification products using magnetic bead aggregation inhibition. A third embodiment provides a platform for simultaneous detection of mRNA markers from blood, cell-free semen, sperm, saliva, and vaginal fluid. The third embodiment comprises a system and method that provide for simple, rapid, and fluorescence-free detection of body fluids using mRNA marker amplification and optical detection for mRNA marker analysis with a smart phone with image analysis.

A target analysis method and target analyzing chip are provided to directly detect a target, such as microRNA, without performing PCR. The target analysis method of the present embodiment includes: a process in which a target in a sample, a labeled probe that binds to the target, and a carrier-binding probe that binds to the target and to a carrier, contact each other, and the target, the labeled probe, and the carrier-binding probe react to bind to each other to form a first bound body; a process in which the first bound body and the carrier contact each other, and the first bound body and the carrier react to bind to each other to form a second bound body; a process to concentrate the carrier; and a process in which the target in the sample is analyzed by detecting a label of the labeled probe bound to the concentrated carrier.

A target analysis method and target analyzing chip are provided to directly detect a target, such as microRNA, without performing PCR. The target analysis method of the present embodiment includes: a process in which a target in a sample, a labeled probe that binds to the target, and a carrier-binding probe that binds to the target and to a carrier, contact each other, and the target, the labeled probe, and the carrier-binding probe react to bind to each other to form a first bound body; a process in which the first bound body and the carrier contact each other, and the first bound body and the carrier react to bind to each other to form a second bound body; a process to concentrate the carrier; and a process in which the target in the sample is analyzed by detecting a label of the labeled probe bound to the concentrated carrier.

The present disclosure provides methods and kits for isolating miRNAs from biological fluids.

The present disclosure provides methods and kits for isolating miRNAs from biological fluids.

The invention described herein provides reagents (e.g., kits), compositions, and methods for carrying out an unbiased genome-wide strategy to identify the functional targets for all ncRNAs.

The invention described herein provides reagents (e.g., kits), compositions, and methods for carrying out an unbiased genome-wide strategy to identify the functional targets for all ncRNAs.

The present invention provides a method for detecting the presence or absence of a target analyte in a test sample, comprising the steps: i) providing a plurality of detector nanoparticles comprising a first detector nanoparticle functionalised with a first probe specific for a first region of a target analyte and a second detector nanoparticle functionalised with a second probe specific for a second region of said target analyte; and ii) contacting said detector nanoparticles with a test sample under conditions suitable for the binding of the specific probes to the target analyte, wherein target analyte-induced agglomeration of the detector nanoparticles permits the release of a detectable signal means from a signal reservoir. Also provided are related devices and systems for performing the method of the invention.

The present invention provides a method for detecting the presence or absence of a target analyte in a test sample, comprising the steps: i) providing a plurality of detector nanoparticles comprising a first detector nanoparticle functionalised with a first probe specific for a first region of a target analyte and a second detector nanoparticle functionalised with a second probe specific for a second region of said target analyte; and ii) contacting said detector nanoparticles with a test sample under conditions suitable for the binding of the specific probes to the target analyte, wherein target analyte-induced agglomeration of the detector nanoparticles permits the release of a detectable signal means from a signal reservoir. Also provided are related devices and systems for performing the method of the invention.

The present invention provides compositions and methods for colorimetric detection schemes for detecting a variety of biomolecules. The compositions and methods employ DNA hybridization chain reaction for catalytic aggregation of gold nanoparticles. In this catalytic aggregation scheme, a single target DNA strand triggers the formation of multiple inter-particle linkages in contrast to the single linkage formed in conventional direct aggregation schemes.