Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

Systems and methods for assessing mRNA in vivo and/or in vitro stability are disclosed. Some embodiments methods obtain RNA indexed or barcoded RNA molecules which are then tested against various conditions including stability inside of cells, stability in cell lysate, and stability in solution (e.g., for storage and/or transportation). Additional embodiments describe methods to determine degradation points with single base resolution.

Systems and methods for assessing mRNA in vivo and/or in vitro stability are disclosed. Some embodiments methods obtain RNA indexed or barcoded RNA molecules which are then tested against various conditions including stability inside of cells, stability in cell lysate, and stability in solution (e.g., for storage and/or transportation). Additional embodiments describe methods to determine degradation points with single base resolution.

Provided herein are methods for simultaneous spatio-temporal measurement of gene expression and cellular activity.

Provided herein are methods for simultaneous spatio-temporal measurement of gene expression and cellular activity.

The present disclosure provides methods and systems for determining a target-activity of at least one resolved oligonucleotide encoded molecule. In an embodiment, a method includes providing a separation medium, wherein the separation medium contains at least one target molecule; and various methods of separating a mixture of at least two oligonucleotide encoded molecules by electrophoresis based on different target-activities of the oligonucleotide encoded molecules for a target molecule. Benefits of the methods disclosed herein can include, without limitation, collecting and calculating qualitative and quantitative data for the target-activity of an encoded portion of the oligonucleotide encoded molecule for a target molecule.

The present disclosure provides methods and systems for determining a target-activity of at least one resolved oligonucleotide encoded molecule. In an embodiment, a method includes providing a separation medium, wherein the separation medium contains at least one target molecule; and various methods of separating a mixture of at least two oligonucleotide encoded molecules by electrophoresis based on different target-activities of the oligonucleotide encoded molecules for a target molecule. Benefits of the methods disclosed herein can include, without limitation, collecting and calculating qualitative and quantitative data for the target-activity of an encoded portion of the oligonucleotide encoded molecule for a target molecule.

The present application describes compositions and methods for identifying and quantitating molecular targets within a cellular environment. Specifically, provided herein are compositions and methods for separately identifying and quantifying each of one or more molecular targets from a single cell. More specifically, provided herein are compositions and methods for separately identifying and quantifying the same molecular target from a single cell.

The present application describes compositions and methods for identifying and quantitating molecular targets within a cellular environment. Specifically, provided herein are compositions and methods for separately identifying and quantifying each of one or more molecular targets from a single cell. More specifically, provided herein are compositions and methods for separately identifying and quantifying the same molecular target from a single cell.

Systems and methods for assessing mRNA in vivo and/or in vitro stability are disclosed. Some embodiments methods obtain RNA indexed or barcoded RNA molecules which are then tested against various conditions including stability inside of cells, stability in cell lysate, and stability in solution (e.g., for storage and/or transportation). Additional embodiments describe methods to determine degradation points with single base resolution.