Disclosed herein are highly sensitive immunoassays that utilize a capture/release mechanism to reduce non-specific binding and achieve detection with attomolar-level sensitivity. Kits that can be used for carrying out these highly sensitive immunoassays are also disclosed herein.

Disclosed herein are highly sensitive immunoassays that utilize a capture/release mechanism to reduce non-specific binding and achieve detection with attomolar-level sensitivity. Kits that can be used for carrying out these highly sensitive immunoassays are also disclosed herein.

This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), the plurality of droplets generated from an aqueous mixture comprising a set of single cells and a set of functionalized particles configured for a single cell assay. Upon generation, the plurality of droplets can be stabilized in position within a region of the collecting container, thereby providing a single-tube workflow for single cell analyses. Further, compositions implemented are structured to allow for overloading of partitions with functionalized particles, such that partitioned single-cells are co-localized with a subset of functionalized particles in a manner that allows for discernable tagging and downstream analyses.

This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), the plurality of droplets generated from an aqueous mixture comprising a set of single cells and a set of functionalized particles configured for a single cell assay. Upon generation, the plurality of droplets can be stabilized in position within a region of the collecting container, thereby providing a single-tube workflow for single cell analyses. Further, compositions implemented are structured to allow for overloading of partitions with functionalized particles, such that partitioned single-cells are co-localized with a subset of functionalized particles in a manner that allows for discernable tagging and downstream analyses.

The present application provides a method and a system for integrating morphological characteristics and gene expression of individual cells. The method comprises the following steps: providing a microfluidic device, which comprises a microwell array and an interdigital electrode, and each microwell comprises a plurality of capture oligonucleotides; injecting cells into the microwells, capturing a single cell and recording morphological characteristics of the cell; lysing the cell so that the mRNA released by the cell is captured by the capture oligonucleotide; reverse transcribing the captured mRNA to obtain cDNA; performing a PCR amplification reaction on the cDNA to obtain a cDNA library and sequencing the cDNA library; reading the cell barcode sequence and the unique molecular identifier sequence according to sequencing results, and the morphological characteristics and gene expression of the cell in the microwell are integrated together.

The present application provides a method and a system for integrating morphological characteristics and gene expression of individual cells. The method comprises the following steps: providing a microfluidic device, which comprises a microwell array and an interdigital electrode, and each microwell comprises a plurality of capture oligonucleotides; injecting cells into the microwells, capturing a single cell and recording morphological characteristics of the cell; lysing the cell so that the mRNA released by the cell is captured by the capture oligonucleotide; reverse transcribing the captured mRNA to obtain cDNA; performing a PCR amplification reaction on the cDNA to obtain a cDNA library and sequencing the cDNA library; reading the cell barcode sequence and the unique molecular identifier sequence according to sequencing results, and the morphological characteristics and gene expression of the cell in the microwell are integrated together.

Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Disclosed herein are point-of-care (POC) diagnostic devices comprising a fibrous carrier, e.g., filter paper, having one or more capture probes configured to display one or more visual outputs indicating the presence of a pathogen in a sample. Also provided herein are methods of using POC diagnostic devices to detect the presence of a pathogen in a sample, e.g., to detect the presence of bacteria in blood.

Disclosed herein are point-of-care (POC) diagnostic devices comprising a fibrous carrier, e.g., filter paper, having one or more capture probes configured to display one or more visual outputs indicating the presence of a pathogen in a sample. Also provided herein are methods of using POC diagnostic devices to detect the presence of a pathogen in a sample, e.g., to detect the presence of bacteria in blood.