Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.

Molecular constructs, populations thereof, arrays, compositions, methods and kits for differentiating a target molecule from an off-target molecule are provided.

Molecular constructs, populations thereof, arrays, compositions, methods and kits for differentiating a target molecule from an off-target molecule are provided.

The present invention relates to a method for detecting and/or characterizing molecular interactions between two molecules, in particular two proteins and most particular between an antigen and an antibody by using an immobilized single-stranded nucleic acid molecule to which single-stranded nucleic acid molecules having a ligand attached thereto are hybridized.

The present invention relates to a method for detecting and/or characterizing molecular interactions between two molecules, in particular two proteins and most particular between an antigen and an antibody by using an immobilized single-stranded nucleic acid molecule to which single-stranded nucleic acid molecules having a ligand attached thereto are hybridized.

Methods and compositions for performing highly sensitive in vitro assays to define substrate preferences and off-target sites of nucleic-acid binding, modifying, and cleaving agents.

Methods and compositions for performing highly sensitive in vitro assays to define substrate preferences and off-target sites of nucleic-acid binding, modifying, and cleaving agents.

A method of base calling nucleobases of two or more polynucleotide sequence portions, wherein said polynucleotide sequence portions have been selectively processed such that an intensity of the signals obtained based upon the respective first nucleobase is greater than an intensity of the signals obtained based upon the respective second nucleobase.

A method of base calling nucleobases of two or more polynucleotide sequence portions, wherein said polynucleotide sequence portions have been selectively processed such that an intensity of the signals obtained based upon the respective first nucleobase is greater than an intensity of the signals obtained based upon the respective second nucleobase.

A method for characterizing a tumor by targeted sequencing includes harvesting genomic DNA from a tumor, sequencing DNA fragments and characterizing the tumor. The sequencing and characterization include hybridizing DNA fragments using a plurality of double-stranded DNA hybridization probes to give hybridized fragments and uniformly enriching simple and complex DNA sequences of the hybridized DNA fragments. The tumor characterization is defined by sequencing target DNA sequences so as to identify genetic aberrations in the tumor.