A method of calibrating a fluorescence in-situ hybridization (FISH) system includes contacting a calibration slide with a plurality of probes, obtaining one or more images of the calibration slide; and calibrating the FISH system based on the one or more images. The calibration slide has a surface with a first plurality of beads. Each bead of the first plurality of beads has one or more binding domains. Each probe has a tag and a targeting domain, and the targeting domain binds a binding domain from the at least one binding domain.

A method of calibrating a fluorescence in-situ hybridization (FISH) system includes contacting a calibration slide with a plurality of probes, obtaining one or more images of the calibration slide; and calibrating the FISH system based on the one or more images. The calibration slide has a surface with a first plurality of beads. Each bead of the first plurality of beads has one or more binding domains. Each probe has a tag and a targeting domain, and the targeting domain binds a binding domain from the at least one binding domain.

A calibration slide for calibrating a multiplexed fluorescence in-situ hybridization (FISH) system has a substrate having a surface and a first plurality of beads on the surface. Each bead of the first plurality of beads has at least one binding domain to be bound to a corresponding probe that has a tag and a targeting domain that binds to a binding domain.

A calibration slide for calibrating a multiplexed fluorescence in-situ hybridization (FISH) system has a substrate having a surface and a first plurality of beads on the surface. Each bead of the first plurality of beads has at least one binding domain to be bound to a corresponding probe that has a tag and a targeting domain that binds to a binding domain.

The present invention relates to methods for the detection of nucleic acids of defined sequence and kits and devices for use in said methods. The methods employ restriction enzymes, polymerase and oligonucleotide primers to produce an amplification product in the presence of a target nucleic acid, which is contacted with oligonucleotide probes to produce a detector product.

The present invention relates to methods for the detection of nucleic acids of defined sequence and kits and devices for use in said methods. The methods employ restriction enzymes, polymerase and oligonucleotide primers to produce an amplification product in the presence of a target nucleic acid, which is contacted with oligonucleotide probes to produce a detector product.

The present invention relates to a method for detecting at least one target nucleic acid sequence from a nucleic acid mixture by a double quenched assay. The double quenched assay of the method exploits a novel approach for melting temperature mediated identification of multiple target nucleic acid sequences. The invention further relates to a kit of parts.

The present invention relates to a method for detecting at least one target nucleic acid sequence from a nucleic acid mixture by a double quenched assay. The double quenched assay of the method exploits a novel approach for melting temperature mediated identification of multiple target nucleic acid sequences. The invention further relates to a kit of parts.

A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.