Embodiments of the present invention relate to apparatus and methods of detecting and/or quantifying a target moiety in a sample comprising the use of a nucleic-acid molecule based recapture event. Particularly although not exclusively certain embodiments of the present invention relate to apparatus and assays which comprise a displacement of an immobilised nucleic acid molecule to form a target-nucleic acid molecule complex and detection of a subsequent recapture event of either the target-nucleic acid complex or the nucleic acid molecule on its own. Other aspects and embodiments are described herein.

Provided herein are devices, systems, kits and methods for predicting or determining the gender of a fetus using cell free fetal nucleic acids in a small amount of maternal biological sample. Devices can be used at point of need during early stages of pregnancy and are compatible with communication devices.

Platforms, including devices, systems, kits, and methods, are provided for the differential detection of Dirofilaria immitis and Dirofilaria. repens in an animal host using parasite-specific DNA target capture techniques as well as polymerase chain reaction detection of those targets.

Provided herein is a cell-free biosensor lateral flow device kit for detection of analytes of interest and methods of using thereof. The device comprises a substrate and a first end, wherein the first end comprises a sample loading portion. The device additionally may comprise a sensor module, wherein the sensor module comprises an allosteric transcription factor regulated in vitro transcription reaction, and a transduction module, wherein the transduction module comprises Bait and Prey nucleic acids which sense the output of the sensor element and a reporter conjugate which accumulates at a test zone when an analyte the sensor element senses is present in the sample.

Provided herein is a cell-free biosensor lateral flow device kit for detection of analytes of interest and methods of using thereof. The device comprises a substrate and a first end, wherein the first end comprises a sample loading portion. The device additionally may comprise a sensor module, wherein the sensor module comprises an allosteric transcription factor regulated in vitro transcription reaction, and a transduction module, wherein the transduction module comprises Bait and Prey nucleic acids which sense the output of the sensor element and a reporter conjugate which accumulates at a test zone when an analyte the sensor element senses is present in the sample.

Described herein in an embodiment is a lateral flow assay strip for detecting the presence of one or more target nucleic acid sequences (e.g., one or more nucleic acid sequences of a pathogen, such as a coronavirus or an influenza virus). The lateral flow assay strip may comprise a first test line configured to detect a first target nucleic acid sequence and a flow control line configured to detect a liquid. In some cases, the first test line comprises a biological capture reagent (e.g., an immobilized antibody). In some cases, the flow control line comprises a non-biological fluid indicator (i.e., a non-antibody reagent). The non-biological fluid indicator may, for example, comprise a pH-sensitive material, a moisture-sensitive material, and/or a chemically-sensitive material. The flow control line may provide a visual indication of whether a fluidic sample was successfully transported from a first end of the lateral flow assay strip to the first test line.

Described herein in an embodiment is a lateral flow assay strip for detecting the presence of one or more target nucleic acid sequences (e.g., one or more nucleic acid sequences of a pathogen, such as a coronavirus or an influenza virus). The lateral flow assay strip may comprise a first test line configured to detect a first target nucleic acid sequence and a flow control line configured to detect a liquid. In some cases, the first test line comprises a biological capture reagent (e.g., an immobilized antibody). In some cases, the flow control line comprises a non-biological fluid indicator (i.e., a non-antibody reagent). The non-biological fluid indicator may, for example, comprise a pH-sensitive material, a moisture-sensitive material, and/or a chemically-sensitive material. The flow control line may provide a visual indication of whether a fluidic sample was successfully transported from a first end of the lateral flow assay strip to the first test line.

Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

A manually actuated chromatography device comprising a chamber for receiving a liquid sample, a pump with a metering valve, and a chromatography element, wherein the pump moves a predetermined volume of liquid from the sample chamber to the chromatography element.