The present disclosure relates to strains for degrading ethylene oxide and degradation agents comprising the same, wherein the strains are Acetobacter peroxydans EO-01 strain with Deposit number of CGMCC No. 18431; Lactobacillus fermentum EO-02 strain with Deposit number of CGMCC No. 18432; or Bacillus subtilis EO-03 strain with Deposit number of CGMCC No. 18433. The strains are capable of safely and efficiently degrading ethylene oxide. The present disclosure also provides a method for purifying and producing strains that can degrade ethylene oxide, and a method for biodegrading ethylene oxide.

The present disclosure relates to strains for degrading ethylene oxide and degradation agents comprising the same, wherein the strains are Acetobacter peroxydans EO-01 strain with Deposit number of CGMCC No. 18431; Lactobacillus fermentum EO-02 strain with Deposit number of CGMCC No. 18432; or Bacillus subtilis EO-03 strain with Deposit number of CGMCC No. 18433. The strains are capable of safely and efficiently degrading ethylene oxide. The present disclosure also provides a method for purifying and producing strains that can degrade ethylene oxide, and a method for biodegrading ethylene oxide.

The present invention provides for a probiotic composition for enhancing a subject's, such as an insect's, resistance to a pesticide comprising: (a) a microorganism capable of expressing one or more enzymes for metabolizing the pesticide, and (b) at least one feed ingredient for a subject. The present invention also provides for a method for enhancing a subject's, such as an insect's, resistance to a pesticide comprising administering to a subject the probiotic composition.

Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.

Provided herein are methods for increasing plant growth, plant productivity and/or soil quality using microbial fertilizers comprising Lactobacillus parafarraginis, Lactobacillus buchneri, Lactobacillus rapi, Lactobacillus zeae, Acetobacter fabarum and Candida ethanolica.

Provided herein are microbial inoculants for use in increasing plant growth, plant productivity and/or soil quality, comprising strains of one or more bacterial species selected from Lactobacillus parafarraginis, Lactobacillus buchneri, Lactobacillus rapi and Lactobacillus zeae. Optionally the microbial inoculants also comprise a strain of Acetobacter fabarum and/or a strain of Candida ethanolica. Also provided are fertilizer compositions comprising said microbial inoculants.

The invention is directed to a process for producing one or more fermentation product in a multi-stage process including an inoculation reactor and at least one bioreactor. The inoculation reactor is fed a C1-containing gaseous substrate containing a reduced amount of hydrogen. The hydrogen is reduced to increase the proportion of CO in the C1-containing gaseous substrate being provided to the inoculation reactor. The inoculation reactor ferments the CO-rich C1-containing gaseous substrate and produces an inoculum, which is fed to at least one bioreactor. The bioreactor receives the C1-containing gaseous substrate, which may or may not contain reduced amounts of hydrogen, to produce one or more fermentation product. By providing a CO-rich C1-containing gaseous substrate to the inoculation reactor, both the inoculation reactor and the subsequent bioreactor(s), are able to have increased stability and product selectivity.

A ketoreductase mutant and use thereof are provided. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO: 1, wherein the mutation at least comprises one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence.

A microbial-based composition provides a co-cultured microorganism consortium in culture medium that includes one or more Acetobacter sp., Bacillus sp., Bifidobacterium sp., Enter ococus sp., Gluconacetobacter sp., Lactobacillus sp., Rhodopseudomonas sp., Saccharomyces sp., Pichia sp., and Trichoderma sp., as well as a carbon source and chlorine-free water. In some embodiments, the microbial-based composition is useful in the agricultural industry as a plant growth promoting and silage-enhancing agent. For plant growth promotion applications, the microbial-based composition may be applied to the foliar surface of a plant or to the plant growth medium, such as soil or hydroponic solution, surrounding the plant. In silage operations, the microbial-based composition may be applied to a cut plant product during one or more stages in the silage process.

A method for inoculating a plant with a nitrogen-fixing bacteria such as Gluconacetobacter diazotrophicus, said method comprising administering the nitrogen-fixing bacteria to a wound of a growing plant, for example to recently cut grass. Inoculation in this manner leads to enhanced growth characteristics including increased greenness of grass. Novel compositions suitable for use in the method are also described and claimed, together with kits for producing these.