Provided is a method which enables efficient separation of a component such as microorganisms from an organic substance-containing liquid obtained by microbial fermentation. Disclosed is a method for producing an organic substance comprising a microbial fermentation step of obtaining an organic substance-containing liquid and a separation step of heating the organic substance-containing liquid and separating into a liquid or solid component containing microorganisms and a gaseous component containing the organic substance.

A system for modifying the genome of Clostridium strains is provided based on a modified endogenous CRISPR array. The application also describes Clostridium strains modified for enhanced butanol production wherein the modified strains are produced using the novel CRISPR-Cas system.

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of eilluent from a chemostat vessel in which a target bacterial ecosystem has been culnn-ed. Methods of using the supplement for culturing or isolating anaerobic microbial strains or cormmmities, particularly anaerobic bacteria from the human gut, are also provided.

Provided are a device for producing an organic substance capable of preventing foaming in a purification section to purify an organic substance and obtaining the organic substance with high yield and a gas treatment system having such a device for producing an organic substance. A device for producing an organic substance, includes: a fermenter 4 configured to generate an organic substance from gas by fermenting action of a microorganism; a separation section 5 configured to separate a discharge fluid discharged from the fermenter 4 into a first liquid containing a solid substance containing the microorganism and the organic substance and a second liquid containing the organic substance and a less content of the solid substance than that in the first liquid; an evaporation section 6 configured to evaporate a liquid component containing the organic substance from the first liquid; and a purification section 7 configured to purify the organic substance from the second liquid and the liquid component vaporized in the evaporation section 6.

Control processes are provided for enhancing biological processes for converting gaseous substrates to useful end products such as ethanol. A process includes providing a gaseous substrate comprising one or more of CO and CO.sub.2 to a bioreactor at a substrate addition rate. Acetogenic bacteria provided to the bioreactor ferment the gaseous substrate in a fermentation broth. The process includes determining a concentration of carboxylic acids and/or carboxylates in the fermentation broth. A control algorithm uses those concentrations to adjust the gaseous substrate addition rate.

A method of producing chemicals includes providing fermentative cells; co-feeding any of galacturonate and galacturonate polymers with carbohydrates to the fermentative cells; and producing a chemical from the fermentative cells. The fermentative cells may include any of Clostridium acetobutylicum and Clostridium saccharoperbutylacetonicum. The carbohydrates may include any of glucose, mannose, galactose, fructose, arabinose, xylose, sucrose, lactose, maltose, cellobiose, and starch. The method may include providing a substantially equal proportion of the any of galacturonate and galacturonate polymers and the carbohydrates for co-feeding to the fermentative cells. The method may include altering a proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The method may include modulating a production of the chemical by altering the proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The chemical may include any of acetate and butyrate.

An object of the present invention is to a method for producing a functional substance utilizing a reaction requiring hydrogen, which production method is safe and efficient; and this object is achieved by a method for producing a functional substance utilizing a reaction requiring hydrogen, the method comprising: supplying the hydrogen by culturing of a hydrogen-producing microorganism.

A Kurthia gibsonii strain EO-06 with Deposit Number of CGMCC No. 18436, a Clostridium kogasensis strain EO-08 with Deposit Number of CGMCC No. 18438 and a Clostridium acidisoli strain EO-09 with Deposit Number of CGMCC No. 18439 are provided. The above strains can be used to treat pollution, for example, to treat industrial gas or wastewater containing ethylene oxide, which greatly improves the decontamination disposal capacity of ethylene oxide in industrial production. The present disclosure also provides a degradation agent for degrading ethylene oxide and a method for biodegrading ethylene oxide.