Provided are an isolated polynucleotide, a host cell including the isolated polynucleotide, and a method of expressing a target gene in the host cell, wherein the isolated polynucleotide includes a promoter region derived from a bacterium of the genus Pseudomonas.

The disclosure relates to isolated microorganismsincluding novel strains of the microorganisms-microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important crop species.

The present invention relates to a co-culture system comprising two different strains of the bacterium Cupriavidus necator (Ralstonia eutropha) and its use for production of biomass and value-added compounds.

In the present invention, microbial inoculants that promoted leafy green plant growth were developed using Rhodococcus erythropolis, Stenotrophomonas maltophilia, Serratia grimesii, and Pseudomonas toyotomiensis. The microbial inoculants include probiotics effective on their own and others effective in specific combinations as follows: Rhodococcus erythropolis; Rhodococcus erythropolis and Serratia grimesii; Serratia grimesii; Serratia grimesii and Pseudomonas toyotomiensis; Pseudomonas toyotomiensis and Stenotrophomonas maltophilia; Rhodococcus erythropolis, Pseudomonas toyotomiensis and Stenotrophomonas maltophilia; Rhodococcus erythropolis, Pseudomonas toyotomiensis, Stenotrophomonas maltophilia, and Serratia grimesii; Shinella zoogloeoides; and Rhodococcus erythropolis, Pseudomonas toyotomiensis, Stenotrophomonas maltophilia, Serratia grimesii and Shinella zoogloeoides. It was found that other probiotics were not effective on their own and there were other combinations that were not effective or inhibitory for plant growth.

A method of preparing a FeMnCeO.sub.x biomaterial is provided, including the following steps. A Pseudomonas sp. strain KW-2 is obtained. A culture medium with a pH of 6.5-7.8 is prepared, which includes 0.1 g/L K.sub.2HPO.sub.4, 0.2 g/L MnSO.sub.4.Math.7H.sub.2O, 0.2 g/L NaNO.sub.3, 0.1 g/L CaCl.sub.2), 0.1 g/L NH.sub.4Cl, 0.1 g/L (NH.sub.4).sub.2CO.sub.3, 35 g/L NaCl and 150 mg/L ferric ammonium citrate. The culture medium is autoclaved, inoculated with the KW-2 strain, cultured for 1-3 days, added with a cerium nitrate solution, cultured for 3-7 days and centrifuged at 4,000-8,000 rpm for 10-20 min to collect a precipitate. The precipitate is rinsed 5-8 times with deionized water and 0.01 mol/L phosphate buffered saline (PBS) and freeze-dried at ?60? C. to obtain the FeMnCeO.sub.x biomaterial. A method for treating antibiotic wastewater using the FeMnCeO.sub.x biomaterial is also provided.

Pseudomonas sp. strain KW-2.

A fermentation method for mupirocin, the method comprising performing shake flask seed culture, seed tank culture, fermentation culture, feed culture. The fermentation method is not only suitable for industrial mass production, but also improves the fermentation potency of mupirocin, which can be stably maintained to be 8000 ug/ml or above.

A Pseudomonas sp. ECO-1 strain was preserved at the China General Microbiological Culture Collection Center (CGMCC) on Mar. 31, 2017, with the preservation number of CGMCC No. 13960. The Pseudomonas sp. ECO-1 strain was separated from POPs (Persistent Organic Pollutants) polluted soil for the first time. The bifunctional enzyme preparation capable of efficiently degrading polychlorinated biphenyl and atrazine was prepared by utilizing the strain for the first time; especially, the bifunctional enzyme preparation has remarkable degradation activity on the polychlorinated biphenyl which is difficult to degrade under an aerobic condition, which is completely different from functions of existing known Pseudomonas sp. and enzyme preparations thereof.

A method of treating a biofilm on a surface, comprising: providing a surface having a biofilm; and administering to the surface a treatment that reduces a concentration of pyruvate of the biofilm, comprising pyruvate produced by at least a portion the biofilm, under conditions effective reducing maintenance of the biofilm on the surface. A composition, comprising purified enzyme, within a particle, effective for reducing pyruvate concentration in an aqueous suspension of the composition.

Methods for increasing carbon-based chemical product yield in an organism by perturbing redox balance in an organism as well as nonnaturally occurring organisms with perturbed redox balance and methods for their use in producing carbon-based chemical products are provided.