The present application relates to plant growth promoting microbes (PGPMs), compositions comprising these PGPMs and methods of using these PGPMs and/or compositions for enhancing plant health, plant growth and/or plant yield, and/or for preventing, inhibiting, or treating the development of plant pathogens or the development of phytopathogenic diseases. This application also provides non-naturally occurring plant varieties that are artificially infected with a PGPM descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.

The methods and systems disclose enzymes that function to import malonic acid or malonates into a cell. The enzymes increase the output of precursor molecules by enriching certain pathways in the cell. The precursor molecules can be converted to cannabinoids. The enzymes are a family of proteins which have a majority of common alignments.

The current invention relates to a purified bacterial strain for improving plant growth and/or yield. The invention also relates to a bacterial population, a microbial active ingredient, an agriculturally active ingredient, a synthetic composition, and methods for improving plant growth and/or yield by improving a trait of agronomic importance in a plant and by conferring resistance to a plant pathogen infection.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present disclosure provides a heteropoly oligosaccharide and an application thereof in improving plant disease resistance. The heteropoly oligosaccharide includes seven D-glucose residues and one D-galactose residue. Each heteropoly oligosaccharide molecule comprises R.sub.1 and R.sub.2, R.sub.1 being H or a monomolecular pyruvate group, R.sub.2 being H or a monomolecular succinyl group, and the structure thereof is represented as follows:

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC13085 and TIC13087. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC13085 and TIC13087 pesticidal proteins are also provided.

The present disclosure discloses an AtPPR1 gene (negative regulatory factor) for Phytophthora resistance and homologous genes thereof, involving an AtPPR1 gene and a protein encoded by the AtPPR1 gene. The AtPPR1 gene has a nucleotide sequence shown in TAIR Gene Locus: At4G02820.1, and the protein encoded by the AtPPR1 gene has an amino acid sequence shown in TAIR Accession AASequence NO.: 1009127979. A function of the AtPPR1 gene of the present disclosure to improve Phytophthora resistance of plants can be used for the selective breeding of Phytophthora-resistant varieties. As a new type of negative immunoregulatory factor in plants, AtPPR1 negatively regulates the resistance of plants to Phytophthora by interfering with downstream signal transduction of endogenous jasmonic acid (JA) and salicylic acid (SA) and ROS signals in plants.