A process is disclosed for production of a polyhydroxyalkanoate that includes depolymerization of a post-consumer polyhydroxyalkanoate and utilization of the hydroxyalkanoate monomer thus produced as a carbon source for a microorganism capable of production of a polyhydroxyalkanoate. Methods can be utilized for true cyclic use of polyhydroxyalkanoates including polyhydroxybutyrates. Various aspects are described including simultaneous depolymerization and polymer production, utilization of purified depolymerase enzymes and/or microorganisms that express a depolymerase in conjunction with a microorganism that produces polymer, utilization of microorganisms that produce both a depolymerase and a new polymer, and utilization of genetically modified organisms to produce natural or modified depolymerase enzymes.

The present disclosure provides engineered bacterial cells having one or more genetic modifications that result in increased production of expression products, such as increased production of plasmids with reduced occurrence of plasmid concatemers or multimers. Also disclosed herein are expression systems for product manufacturing in media having reduced antibiotic concentration, as well as methods of making cells for use in the expression systems. The cells and expression systems increase the growth rate and production yields of biomolecules produced within the host bacterial host cells without the use of antibiotics, and can produce the product with little to no endotoxins present.

This disclosure provides methods and genetically engineered strains of Vibrio natriegens, specifically developed for the bio-based production of succinate. Capitalizing on the rapid growth kinetics and highly efficient carbon metabolism of V. natriegens, this disclosure provides an environmentally friendly, scalable, and cost-effective alternative to traditional petrochemical methods for succinate production.

The present disclosure relates to a process for producing biogenic crystals of calcium carbonate, said process is remark-able in that it comprises the following steps: a) providing one or more colonies of CaCO3-producing bacteria; b) inoculating said one or more colonies in a first marine broth under inoculating conditions to form an inoculated culture; c) adding a second marine broth to the inoculated culture to form a dissolved inoculated culture and/or collecting a supernatant of the inoculated culture and adding a second marine broth to said supernatant; d) incubating said dissolved inoculated culture and/or said supernatant cell culture under incubating conditions to form a mixture of biogenic crystals of calcium carbonate, optionally, recovering said mixture of biogenic crystals of calcium carbonate.

The present disclosure relates, in part, to microbial hosts capable of synthesizing cis-3-hexenol, cis-3-hexenal, trans-3-hexenol, trans-3-hexenal, trans-2-hexenal, cis-2-hexenal and related compounds from hexanoic acid and methods for the preparation of cis-3-hexenol, cis-3-hexenal, trans-3-hexenol, trans-3-hexenal, trans-2-hexenal, cis-2-hexenal and related compounds.

In alternative embodiments, provided are compositions, including products of manufacture and kits, and methods, for producing enhanced marine bacterial strains with the ability to produce stimulatory products that amplify their probiotic effects for reef restoration and biotechnology applications. In alternative embodiments, genetically engineered marine bacteria (such as Nereida, Vibrio, Pseudoalteromonas and/or Roseobacter bacterium) as provided herein, and compositions, products of manufacture, kits and methods as provided herein, are used in biomaterial production, probiotic use in aquaculture and/or for environmental restoration purposes.